Rabbit DAD1 Polyclonal Antibody | anti-DAD1 antibody

DAD1 Antibody (C-term)

Cat. Num. AAA287237

• Gene Names: DAD1; OST2
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: DAD1, Antibody; DAD1 Antibody (C-term); Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Oligosaccharyl transferase subunit DAD1; Defender against cell death 1; DAD-1; DAD1; anti-DAD1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Pig, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This DAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the C-terminal region of human DAD1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 64-92
• Sequence Length: 113

• Applicable Applications for anti-DAD1 antibody: ELISA, WB (Western Blot)
• Function: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853, PubMed:31831667). N- glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B- containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853).
• Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(DAD1 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DAD1 antibody detected the DAD1 protein (arrow).)

Related Product Information for anti-DAD1 antibody

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq].

Product Categories/Family for anti-DAD1 antibody

Cell Biology

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kulke, M.H., et al. Genes Chromosomes Cancer 47(7):591-603(2008)
Shibatani, T., et al. Biochemistry 44(16):5982-5992(2005)

NCBI and Uniprot Product Information

• NCBI GI #: 4503253
• NCBI GeneID: 1603
• NCBI Accession #: NP_001335.1
• NCBI GenBank Nucleotide #: NM_001344.3
• UniProt Accession #: P61803; O08552; O70364; P46966; P46968; Q6FGA3; Q96GB7; D3DS25
• Molecular Weight: 12497
• NCBI Official Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• NCBI Official Synonym Full Names: defender against cell death 1
• NCBI Official Symbol: DAD1
• NCBI Official Synonym Symbols: OST2
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• UniProt Gene Name: DAD1
• UniProt Synonym Gene Names: Oligosaccharyl transferase subunit DAD1; DAD-1
• UniProt Entry Name: DAD1_HUMAN