IHC (Immunohistochemisry)
(Immunofluorescent analysis of HepG2 cells using AAA229793 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Rabbit anti-Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 Polyclonal Antibody | anti-DAD1 antibody
Rabbit anti-human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 polyclonal Antibody
Gene Names
DAD1; OST2
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Antibody; Rabbit anti-human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 polyclonal Antibody; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein; Defender against cell death 1; anti-DAD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
113
Applicable Applications for anti-DAD1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-12173 / sc-25557
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HepG2 cells using AAA229793 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohistochemisry)
(Immunofluorescent analysis of HepG2 cells using AAA229793 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human prostate using AAA229793 at dilution of 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human prostate using AAA229793 at dilution of 1:100)
WB (Western Blot)
(All lanes: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1 antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12 .4kDaObserved band size: 12.4kDa)
WB (Western Blot)
(All lanes: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit DAD1 antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12 .4kDaObserved band size: 12.4kDa)
Related Product Information for anti-DAD1 antibody
Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.
Product Categories/Family for anti-DAD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,497 Da
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
NCBI Official Synonym Full Names
defender against cell death 1
NCBI Official Symbol
DAD1
NCBI Official Synonym Symbols
OST2
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Protein Name
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
UniProt Gene Name
DAD1
UniProt Synonym Gene Names
Oligosaccharyl transferase subunit DAD1; DAD-1
UniProt Entry Name
DAD1_HUMAN
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Product Notes
The DAD1 dad1 (Catalog #AAA229793) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the DAD1 dad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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