IF (Immunofluorescence)
(Figure 2. IF analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (AAA127063) and anti-Beta Tubulin antibody (M01857-3).DAX-1/NR0B1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-DAX-1/NR0B1 Antibody (AAA127063) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit anti-Human DAX-1/NR0B1 Polyclonal Antibody | anti-DAX-1/NR0B1 antibody
Anti-DAX-1/NR0B1 Antibody Picoband
Gene Names
NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Immunogen affinity purified.
Synonyms
DAX-1/NR0B1, Antibody; Anti-DAX-1/NR0B1 Antibody Picoband; Platelet endothelial cell adhesion molecule; natural cytotoxicity triggering receptor 1; Natural cytotoxicity triggering receptor 1; Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; NKp46; hNKp46; CD335; NCR1; LY94; anti-DAX-1/NR0B1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Selectively expressed by both resting and activated NK cells.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-DAX-1/NR0B1 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA
Immunogen
E coli-derived human DAX-1/NR0B1 recombinant protein (Position: V126-Q404).
Subcellular Localization
Cell membrane, Single-pass type I membrane protein
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (AAA127063) and anti-Beta Tubulin antibody (M01857-3).DAX-1/NR0B1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-DAX-1/NR0B1 Antibody (AAA127063) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (AAA127063) and anti-Beta Tubulin antibody (M01857-3).DAX-1/NR0B1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-DAX-1/NR0B1 Antibody (AAA127063) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (AAA127063).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human U87 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAX-1/NR0B1 antigen affinity purified polyclonal antibody (#AAA127063) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DAX-1/NR0B1 at approximately 48 kDa. The expected band size for DAX-1/NR0B1 is at 48 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (AAA127063).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human U87 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAX-1/NR0B1 antigen affinity purified polyclonal antibody (#AAA127063) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DAX-1/NR0B1 at approximately 48 kDa. The expected band size for DAX-1/NR0B1 is at 48 kDa.)
Related Product Information for anti-DAX-1/NR0B1 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-DAX-1/NR0B1 antibody
References
1. Achermann, J. C., Gu, W.-X., Kotlar, T. J., Meeks, J. J., Sabacan, L. P., Seminara, S. B., Habiby, R. L., Hindmarsh, P. C., Bick, D. P., Sherins, R. J., Crowley, W. F., Jr., Layman, L. C., Jameson, J. L. Mutational analysis of DAX1 in patients with hypogonadotropic hypogonadism or pubertal delay. J. Clin. Endocr. Metab. 84: 4497-4500, 1999. 2. Achermann, J. C., Ito, M., Ito, M., Hindmarsh, P. C., Jameson, J. L. A mutation in the gene encoding steroidogenic factor-1 causes XY sex reversal and adrenal failure in humans. (Letter) Nature Genet. 22: 125-126, 1999.3. Achermann, J. C., Silverman, B. L., Habiby, R. L., Jameson, J. L. Presymptomatic diagnosis of X-linked adrenal hypoplasia congenita by analysis of DAX1. J. Pediat. 137: 878-881, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
470
NCBI Official Full Name
Nuclear receptor subfamily 0 group B member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 0, group B, member 1
NCBI Official Symbol
NR0B1
NCBI Official Synonym Symbols
AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
NCBI Protein Information
nuclear receptor subfamily 0 group B member 1; nuclear receptor DAX-1; nuclear hormone receptor; DSS-AHC critical region on the X chromosome protein 1
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 1
UniProt Gene Name
NR0B1
UniProt Synonym Gene Names
AHC; DAX1
UniProt Entry Name
NR0B1_HUMAN
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Product Notes
The DAX-1/NR0B1 nr0b1 (Catalog #AAA127063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DAX-1/NR0B1 Antibody Picoband reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DAX-1/NR0B1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA. Researchers should empirically determine the suitability of the DAX-1/NR0B1 nr0b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAX-1/NR0B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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