IF (Immunofluorescence)
(Immunofluorescence staining of NR0B1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NR0B1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
Rabbit anti-Human Dax1 Polyclonal Antibody | anti-NR0B1 antibody
Anti-Dax1 Antibody, Rabbit Polyclonal
Gene Names
NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
Dax1, Antibody; Anti-Dax1 Antibody, Rabbit Polyclonal; NR0B1/DAX1 Antibody, Rabbit PAb, Antigen Affinity Purified; nuclear receptor subfamily 0, group B, member 1; Anti-AHC Antibody; Anti-AHCH Antibody; Anti-AHX Antibody; Anti-DAX-1 Antibody; Anti-DAX1 Antibody; Anti-DSS Antibody; Anti-GTD Antibody; Anti-HHG Antibody; Anti-NROB1 Antibody; Anti-SRXY2 Antibody; anti-NR0B1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NR0B1/DAX1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NR0B1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
A synthetic peptide corresponding to the center region of the Human NR0B1/DAX1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human NR0B1/DAX1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of NR0B1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NR0B1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of NR0B1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NR0B1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human NR0B1 in human small intestine with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human NR0B1 in human small intestine with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human NR0B1 in human testis with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human NR0B1 in human testis with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-NR0B1 antibody
The nuclear receptor subfamily 0, group B, member 1 (NR0B1) gene is an orphan nuclear receptor that is X-linked in eutherian mammals and plays a critical role in the establishment and function of the hypothalamic-pituitary-adrenal-gonadal axis. DAX1 (NR0B1) is an orphan nuclear receptor, which plays an important role in the development and function of the adrenal glands and gonads. Mutations in DAX1 cause X-linked adrenal hypoplasia congenita (X-linked AHC), which is characterized by adrenal insufficiency (AI) and hypogonadotropic hypogonadism (HHG).NR0B1, an orphan nuclear receptor, is expressed in side population cells and its knockdown reduces tumorigenic and anti-apoptotic potential in lung adenocarcinoma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
470
NCBI Official Full Name
Nuclear receptor subfamily 0 group B member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 0, group B, member 1
NCBI Official Symbol
NR0B1
NCBI Official Synonym Symbols
AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
NCBI Protein Information
nuclear receptor subfamily 0 group B member 1; nuclear receptor DAX-1; nuclear hormone receptor; DSS-AHC critical region on the X chromosome protein 1
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 1
UniProt Gene Name
NR0B1
UniProt Synonym Gene Names
AHC; DAX1
UniProt Entry Name
NR0B1_HUMAN
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Product Notes
The NR0B1 nr0b1 (Catalog #AAA254464) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Dax1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dax1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NR0B1 nr0b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dax1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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