FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-DBT antibody (AAA128210).Overlay histogram showing SiHa cells stained with AAA128210 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBT Antibody (AAA128210, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit DBT Polyclonal Antibody | anti-DBT antibody
Anti-DBT Antibody Picoband
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-DBT antibody (AAA128210).Overlay histogram showing SiHa cells stained with AAA128210 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBT Antibody (AAA128210, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of SiHa cells using anti-DBT antibody (AAA128210).Overlay histogram showing SiHa cells stained with AAA128210 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DBT Antibody (AAA128210, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
IP (Immunoprecipitation)
(Figure 3. Immunoprecipitating DBT in A431 whole cell lysate.Western blot analysis of DBT using anti-DBT antibody (AAA128210).Lane 1: A431 whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-DBT antibody in A431 whole cell lysate,Lane 3: anti-DBT antibody (2ug) + A431 whole cell lysate (500ug).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DBT antigen affinity purified polyclonal antibody (AAA128210) at a dilution of 0.5ug/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.)
IP (Immunoprecipitation)
(Figure 3. Immunoprecipitating DBT in A431 whole cell lysate.Western blot analysis of DBT using anti-DBT antibody (AAA128210).Lane 1: A431 whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-DBT antibody in A431 whole cell lysate,Lane 3: anti-DBT antibody (2ug) + A431 whole cell lysate (500ug).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DBT antigen affinity purified polyclonal antibody (AAA128210) at a dilution of 0.5ug/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.)
IF (Immunofluorescence)
(Figure 2. IF analysis of DBT using anti-DBT antibody (AAA128210) and anti-Beta Tubulin antibody (M01857-3).DBT was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-DBT Antibody (AAA128210) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of DBT using anti-DBT antibody (AAA128210) and anti-Beta Tubulin antibody (M01857-3).DBT was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-DBT Antibody (AAA128210) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of DBT using anti-DBT antibody (AAA128210).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SiHa whole cell lysates,Lane 2: human A431 whole cell lysates,Lane 3: human Hacat whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat stomach tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse stomach tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBT antigen affinity purified polyclonal antibody (AAA128210) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of DBT using anti-DBT antibody (AAA128210).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SiHa whole cell lysates,Lane 2: human A431 whole cell lysates,Lane 3: human Hacat whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: rat stomach tissue lysates,Lane 6: rat kidney tissue lysates,Lane 7: mouse stomach tissue lysates,Lane 8: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBT antigen affinity purified polyclonal antibody (AAA128210) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DBT at approximately 53 kDa. The expected band size for DBT is at 53 kDa.)
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Product Notes
The DBT dbt (Catalog #AAA128210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DBT Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DBT can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DBT dbt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DBT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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