Rabbit DDIT3 Polyclonal Antibody | anti-DDIT3 antibody

DDIT3 Antibody

Cat. Num. AAA303382

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: DDIT3, Antibody; DDIT3 Antibody; DDIT3; CEBPZ; CHOP; CHOP10; GADD153; anti-DDIT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DDIT3 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-DDIT3 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human DDIT3.
• Target Name: DDIT3
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of A549 cell using DDIT3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded rat kidney using DDIT3 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded mouse spleen using DDIT3 antibody at dilution of 1:100 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts from mouse craniofacial tissue, using DDIT3 antibody.)

Related Product Information for anti-DDIT3 antibody

DDIT3 was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). DDIT3 expression is induced by certain cellular stresses including starvation and the induced DDIT3 suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of DDIT3 expression is elevated and DDIT3 functions to mediate programmed cell death (3). Studies also found that DDIT3 mediates the activation of GADD34 and Ero1-La expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2a thereby stimulating protein synthesis. Ero1-La promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of DDIT3 in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).

Product Categories/Family for anti-DDIT3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 304282225
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181982.1
• NCBI GenBank Nucleotide #: NM_001195053.1
• UniProt Accession #: P35638; F8VS99
• Molecular Weight: 19KD
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 1
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN