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IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DGCR8 antibody . Western blot was performed from the immunoprecipitate using DGCR8 antibody at a dilition of 1:1000.)
Rabbit DGCR8 Polyclonal Antibody | anti-DGCR8 antibody
DGCR8 Rabbit pAb
Gene Names
DGCR8; Gy1; pasha; DGCRK6; C22orf12
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity purification
Synonyms
DGCR8; Polyclonal Antibody; DGCR8 Rabbit pAb; C22orf12; DGCRK6; Gy1; pasha; microprocessor complex subunit DGCR8; anti-DGCR8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-DGCR8 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50-1:200
IHC: 1:50-1:200
IP: 1:50-1:200
Immunogen
Recombinant protein of human DGCR8.
Cellular Location
Nucleus, nucleolus
Positive Samples
HepG2, HeLa, 293T, Mouse testis
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DGCR8 antibody . Western blot was performed from the immunoprecipitate using DGCR8 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DGCR8 antibody . Western blot was performed from the immunoprecipitate using DGCR8 antibody at a dilition of 1:1000.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat ovary using DGCR8 Rabbit pAb at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using DGCR8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using DGCR8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Related Product Information for anti-DGCR8 antibody
Background: This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86045 MW
NCBI Official Full Name
microprocessor complex subunit DGCR8 isoform 2
NCBI Official Synonym Full Names
DGCR8, microprocessor complex subunit
NCBI Official Symbol
DGCR8
NCBI Official Synonym Symbols
Gy1; pasha; DGCRK6; C22orf12
NCBI Protein Information
microprocessor complex subunit DGCR8
UniProt Protein Name
Microprocessor complex subunit DGCR8
UniProt Gene Name
DGCR8
UniProt Synonym Gene Names
C22orf12; DGCRK6
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Product Notes
The DGCR8 dgcr8 (Catalog #AAA28373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGCR8 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DGCR8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the DGCR8 dgcr8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGCR8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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