WB (Western Blot)
(Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody AAA210733 (lot AAA210733-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human DNMT1 Polyclonal Antibody | anti-DNMT1 antibody
Rabbit anti-DNMT1 Antibody, Affinity Purified
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DNMT1, Antibody; Rabbit anti-DNMT1 Antibody, Affinity Purified; DNA methyltransferase HsaI; MCMT; m.HsaI; HSN1E; Dnmt1; ADCADN; DNA MTase HsaI; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5-)-methyltransferase 1; CXXC-type zinc finger protein 9; CXXC9; AIM; DNMT; DNA methyltransferase 1; anti-DNMT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DNMT1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 700 and 750
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody AAA210733 (lot AAA210733-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human DNMT1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DNMT1 antibody AAA210733 (lot AAA210733-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody AAA210733 (lot AAA210733-2) used for IP at 3 ug per reaction. For blotting immunoprecipitated DNMT1, AAA210733 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human DNMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DNMT1 antibody AAA210733 (lot AAA210733-2) used for IP at 3 ug per reaction. For blotting immunoprecipitated DNMT1, AAA210733 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-DNMT1 antibody
DNA methyltransferase 1 (DNMT1) catalyzes the methylation of the 5'-cytosine in the dinucleotide sequence, CpG. DNMT1 is thought to be responsible for the regulation of methylation patterns that are important in development and influence gene expression in a time- and tissue-specific manner.
Product Categories/Family for anti-DNMT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
144,465 Da
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform b
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 1
NCBI Official Symbol
DNMT1
NCBI Official Synonym Symbols
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Gene Name
DNMT1
UniProt Synonym Gene Names
AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
UniProt Entry Name
DNMT1_HUMAN
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Product Notes
The DNMT1 dnmt1 (Catalog #AAA210733) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DNMT1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNMT1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DNMT1 dnmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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