IP (Immunoprecipitation)
(DNAJC24 was immunoprecipitated using:Lane A:0.5 mg HL-60 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate4 uL anti-DNAJC24 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DNAJC24 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 26 kDa)
Rabbit anti-Human DPH4/DNAJC24 Polyclonal Antibody | anti-DNAJC24 antibody
Anti-DPH4/DNAJC24 Antibody, Rabbit Polyclonal
Gene Names
DNAJC24; DPH4; JJJ3; ZCSL3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DPH4/DNAJC24, Antibody; Anti-DPH4/DNAJC24 Antibody, Rabbit Polyclonal; DNAJC24 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-AC108456.1 Antibody; Anti-JJJ3 Antibody; Anti-ZCSL3 Antibody; anti-DNAJC24 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DNAJC24
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DNAJC24 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human DNAJC24
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DNAJC24, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(DNAJC24 was immunoprecipitated using:Lane A:0.5 mg HL-60 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate4 uL anti-DNAJC24 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DNAJC24 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 26 kDa)
IP (Immunoprecipitation)
(DNAJC24 was immunoprecipitated using:Lane A:0.5 mg HL-60 Whole Cell LysateLane B:0.5 mg Raw264.7 Whole Cell Lysate4 uL anti-DNAJC24 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DNAJC24 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 26 kDa)
WB (Western Blot)
(Anti-DNAJC24 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:26 kDa)
WB (Western Blot)
(Anti-DNAJC24 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:26 kDa)
Related Product Information for anti-DNAJC24 antibody
Diphthamide is a post-translationally modified histidine residue found in eukaryotic and archaeal translation elongation factor 2 (EF2). It is named after the fact that it is the target of diphtheria toxin (DT) produced by Corynebacterium diphtheriae. DT and the Pseudomonas aeruginosa exotoxin A (ETA) catalyze the ADP-ribosylation reaction on the diphthamide residue of EF2 using nicotinamide adenine dinucleotide (NAD) as the ADP-ribosyl donor. EF2 catalyzes the translocation of mRNA and peptidyl-tRNA from ribosome A site to P site. The ADP-ribosylation of EF2 inactivates it, stopping protein synthesis and leading to cell death. This modification is conserved from archaebacteria to humans. DPH4 (also known as DNAJC24, JJJ3 and ZCSL3) is 1 of several enzymes involved in synthesis of diphthamide in EEF2.
References
Su X, et al. (2013) The biosynthesis and biological function of diphthamide. Crit Rev Biochem Mol Biol 48 (6): 515-521.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,693 Da
NCBI Official Full Name
dnaJ homolog subfamily C member 24
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily C, member 24
NCBI Official Symbol
DNAJC24
NCBI Official Synonym Symbols
DPH4; JJJ3; ZCSL3
NCBI Protein Information
dnaJ homolog subfamily C member 24; 1700030A21Rik; CSL-type zinc finger-containing protein 3; DPH4 homolog (JJJ3, S. cerevisiae); DPH4, JJJ3 homolog; diphthamide biosynthesis protein 4; zinc finger, CSL domain containing 3; zinc finger, CSL-type containin
UniProt Protein Name
DnaJ homolog subfamily C member 24
UniProt Gene Name
DNAJC24
UniProt Synonym Gene Names
DPH4; ZCSL3
UniProt Entry Name
DJC24_HUMAN
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Product Notes
The DNAJC24 dnajc24 (Catalog #AAA254772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DPH4/DNAJC24 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPH4/DNAJC24 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DNAJC24 dnajc24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPH4/DNAJC24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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