WB (Western Blot)
(Applications: Western BlotSample: Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/laneDetection: SuperSignal West Pico Chemiluminescent Substrate"Rabbit antibody AAA45743 from was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this antibody for detection of EGFR with the intact N-terminal domain.")
Rabbit anti-Human, Rat EGFR Polyclonal Antibody | anti-EGFR antibody
Anti-EGFR antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
EGFR, Antibody; Anti-EGFR antibody; Epidermal growth factor receptor; epidermal growth factor receptor; Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody; Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody; Cell growth inhibiting protein 40 antibody; Cell proliferation inducing protein 61 antibody; EGF R antibody; EGFR antibody; EGFR_HUMAN antibody; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody; Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody; Epidermal growth factor receptor antibody; erbb 1 antibody; Erbb antibody; Erbb1 antibody; ERBB1 antibody; Errp antibody; HER1 antibody; mENA antibody; Oncogene ERBB antibody; PIG61 antibody; Proto-oncogene c-ErbB-1 antibody; Receptor tyrosine protein kinase ErbB 1 antibody; Receptor tyrosine protein kinase ErbB1 antibody; Receptor tyrosine-protein kinase ErbB-1 antibody; Urogastrone antibody; wa2 antibody; Wa5 antibody; anti-EGFR antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
1210
Applicable Applications for anti-EGFR antibody
WB (Western Blot)
Immunogen
E Coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Applications: Western BlotSample: Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/laneDetection: SuperSignal West Pico Chemiluminescent Substrate"Rabbit antibody AAA45743 from was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this antibody for detection of EGFR with the intact N-terminal domain.")
WB (Western Blot)
(Applications: Western BlotSample: Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/laneDetection: SuperSignal West Pico Chemiluminescent Substrate"Rabbit antibody AAA45743 from was probed for western blot detection of the recombinant N-terminal domain of human EGFR (100 ng/lane). The antibody nicely detected the protein band with the expected mol.wt. of recombinant protein. The antibody appears to be specific since non-specific bands were not detected in parallel lanes with cell lysates lacking EGFR. I would highly recommend this antibody for detection of EGFR with the intact N-terminal domain.")
Application Data
(Anti-EGFR Picoband antibody, AAA45743-1.jpgAll lanes: Anti-EGFR(AAA45743) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: A431 Whole Cell Lysate at 40ugPredicted bind size: 134KDObserved bind size: 200KD)
Application Data
(Anti-EGFR Picoband antibody, AAA45743-1.jpgAll lanes: Anti-EGFR(AAA45743) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: A431 Whole Cell Lysate at 40ugPredicted bind size: 134KDObserved bind size: 200KD)
Related Product Information for anti-EGFR antibody
Description: Rabbit IgG polyclonal antibody for Epidermal growth factor receptor(EGFR) detection. Tested with WB in Human, Rat.
Background: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
Background: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
69,228 Da
NCBI Official Full Name
Epidermal growth factor receptor
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
NCBI Protein Information
epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA45743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EGFR antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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