IF (Immunofluorescence)
Sheep EGFR Polyclonal Antibody | anti-EGFR antibody
EGFR antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
Synonyms
EGFR, Antibody; EGFR antibody; Polyclonal EGFR; Anti-EGFR; v-erb-b homolog; Epidermal Growth FactorEgf receptor; egf-r; panitumumab; egfr; epidermal growth factor receptor; erbb1; anti-EGFR antibody
Host
Sheep
Clonality
Polyclonal
Specificity
Human
Form/Format
Protein G Purified Ig Fraction in 0.15M PBS buffer, pH 7.4 with no preservatives added.
Concentration
2.2mg/ml (varies by lot)
Sequence Length
1167
Applicable Applications for anti-EGFR antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry)
Cross Reactivity
Recognizes human, rat, mouse chicken receptors. Tested against the A-431 epidermal carcinoma. This lot of antibody has superior binding characteristics for immuno-precipitation and has been useful in Western Blot, IF, EIA, and Formalin fixed cell immuno-cytochemistry.
Biological Significance
The epidermal growth factor receptor (EGFR) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGF alpha).
Cross-Reactivity
Mouse, rat, turkey
Immunogen
Recombinant 20a.a. residues representing a portion of the internal domain of the receptor proximal to phosphorylation region.
Preparation and Storage
4°C short term only, for long term storage, aliquot and freeze at -20°C.
IF (Immunofluorescence)
IF (Immunofluorescence)
Related Product Information for anti-EGFR antibody
Sheep polyclonal EGFR antibody
Product Categories/Family for anti-EGFR antibody
References
Cell adhesion and EGFR activation regulate EphA2 expression in cancer; Alice Bjerregaard Larsen, Marie-Th©r©se Stockhausen, Hans Skovgaard Poulsen (Cellular Signalling: Volume: 22 Issue: 4 Page: 636-644 DOI: 10.1016/j.cellsig.2009.11.018)
Identification of 14-3-3 zeta as an EGF receptor interacting protein; Morten P Oksvolda, Henrik S Huitfeldtb, Wallace Y Langdon (FEBS Letters: Volume: 569 Issue: 1-3 Page: 207-210 DOI: 10.1016/j.febslet.2004.05.068)
The Cbl-interacting protein TULA inhibits dynamin-dependent endocytosis; Vibeke Bertelsena, Kamilla Breena, Kirsten Sandvigb, Espen Stanga, Inger Helene Madshus (Experimental Cell Research: Volume: 313 Issue: 8 Page: 1696-1709 DOI: 10.1016/j.yexcr.2007.02.017)
Dysregulation of Ack1 inhibits down-regulation of the EGF receptor; Lene Mels¦ther Gr¸vdal, Lene E. Johannessen, Marianne Skeie R¸dland, Inger Helene Madshus, Espen Stang (Experimental Cell Research: Volume: 314 Issue: 6 Page: 1292-1300 DOI: 10.1016/j.yexcr.2007.12.017)
Maintenance of EGFR and EGFRvIII expressions in an in vivo and in vitro model of human glioblastoma multiforme; Marie-Th©r©se Stockhausen, Helle Broholm, Mette Villingsh¸j, Maria Kirchhoff, Tommy Gerdes, Karina Kristoffersen, Michael Kosteljanetz, Mogens Spang-Thomsen, Hans Skovgaard Poulsen (Experimental Cell Research: Volume: 317 Issue: 11 Page: 1513-1526 DOI: 10.1016/j.yexcr.2011.04.001)
Identification of 14-3-3 zeta as an EGF receptor interacting protein; Morten P Oksvolda, Henrik S Huitfeldtb, Wallace Y Langdon (FEBS Letters: Volume: 569 Issue: 1-3 Page: 207-210 DOI: 10.1016/j.febslet.2004.05.068)
The Cbl-interacting protein TULA inhibits dynamin-dependent endocytosis; Vibeke Bertelsena, Kamilla Breena, Kirsten Sandvigb, Espen Stanga, Inger Helene Madshus (Experimental Cell Research: Volume: 313 Issue: 8 Page: 1696-1709 DOI: 10.1016/j.yexcr.2007.02.017)
Dysregulation of Ack1 inhibits down-regulation of the EGF receptor; Lene Mels¦ther Gr¸vdal, Lene E. Johannessen, Marianne Skeie R¸dland, Inger Helene Madshus, Espen Stang (Experimental Cell Research: Volume: 314 Issue: 6 Page: 1292-1300 DOI: 10.1016/j.yexcr.2007.12.017)
Maintenance of EGFR and EGFRvIII expressions in an in vivo and in vitro model of human glioblastoma multiforme; Marie-Th©r©se Stockhausen, Helle Broholm, Mette Villingsh¸j, Maria Kirchhoff, Tommy Gerdes, Karina Kristoffersen, Michael Kosteljanetz, Mogens Spang-Thomsen, Hans Skovgaard Poulsen (Experimental Cell Research: Volume: 317 Issue: 11 Page: 1513-1526 DOI: 10.1016/j.yexcr.2011.04.001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
epidermal growth factor receptor
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA74912) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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