FCM/FACS (Flow Cytometry)
(EGFR-S1026 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human EGFR-S1026 Polyclonal Antibody | anti-EGFR antibody
EGFR-S1026 Antibody (C-term)
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EGFR-S1026, Antibody; EGFR-S1026 Antibody (C-term); Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB; ERBB1; HER1; anti-EGFR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EGFR-S1026 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1004-1033 amino acids from the C-terminal region of human EGFR-S1026.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1004-1033
Sequence Length
628
Applicable Applications for anti-EGFR antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(EGFR-S1026 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(EGFR-S1026 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(EGFR-S1026 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the EGFR-S1026 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(EGFR-S1026 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the EGFR-S1026 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from A431, HUVEC, PC-3 cell line (from left to right), using EGFR Antibody (pS1026). AAA284808 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from A431, HUVEC, PC-3 cell line (from left to right), using EGFR Antibody (pS1026). AAA284808 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-EGFR antibody
The protein encoded by this gene is a transmembrane
glycoprotein that is a member of the protein kinase superfamily.
This protein is a receptor for members of the epidermal growth
factor family. EGFR is a cell surface protein that binds to
epidermal growth factor. Binding of the protein to a ligand induces
receptor dimerization and tyrosine autophosphorylation and leads to
cell proliferation. Mutations in this gene are associated with lung
cancer.
glycoprotein that is a member of the protein kinase superfamily.
This protein is a receptor for members of the epidermal growth
factor family. EGFR is a cell surface protein that binds to
epidermal growth factor. Binding of the protein to a ligand induces
receptor dimerization and tyrosine autophosphorylation and leads to
cell proliferation. Mutations in this gene are associated with lung
cancer.
Product Categories/Family for anti-EGFR antibody
References
Perez, C.A., et al. J. Urol. 183(5):2062-2069(2010)
Koumakpayi, I.H., et al. Br. J. Cancer 102(7):1163-1173(2010)
Cortot, A.B., et al. Cancer (2010) In press :
Lee, Y.J., et al. J. Cancer Res. Clin. Oncol. (2010) In press :
Kawahara, A., et al. Hum. Pathol. (2010) In press :
Wu, S.L., et al. Mol. Cell Proteomics 5(9):1610-1627(2006)
Wu, S.L., et al. J. Proteome Res. 4(4):1155-1170(2005)
Abe, Y., et al. J. Biol. Chem. 273(18):11150-11157(1998)
Li, W., et al. Mol. Cell. Biol. 12(12):5824-5833(1992)
Krieg, J., et al. J. Biol. Chem. 267(27):19258-19265(1992)
Lowenstein, E.J., et al. Cell 70(3):431-442(1992)
Chi, D.D., et al. Hum. Mol. Genet. 1 (2), 135 (1992) :
Countaway, J.L., et al. J. Biol. Chem. 267(2):1129-1140(1992)
Koumakpayi, I.H., et al. Br. J. Cancer 102(7):1163-1173(2010)
Cortot, A.B., et al. Cancer (2010) In press :
Lee, Y.J., et al. J. Cancer Res. Clin. Oncol. (2010) In press :
Kawahara, A., et al. Hum. Pathol. (2010) In press :
Wu, S.L., et al. Mol. Cell Proteomics 5(9):1610-1627(2006)
Wu, S.L., et al. J. Proteome Res. 4(4):1155-1170(2005)
Abe, Y., et al. J. Biol. Chem. 273(18):11150-11157(1998)
Li, W., et al. Mol. Cell. Biol. 12(12):5824-5833(1992)
Krieg, J., et al. J. Biol. Chem. 267(27):19258-19265(1992)
Lowenstein, E.J., et al. Cell 70(3):431-442(1992)
Chi, D.D., et al. Hum. Mol. Genet. 1 (2), 135 (1992) :
Countaway, J.L., et al. J. Biol. Chem. 267(2):1129-1140(1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134277
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA284808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1004-1033. The EGFR-S1026 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR-S1026 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR-S1026, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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