WB (Western Blot)
(Detection of human eIF6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF6 antibody AAA212098 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
Rabbit anti-Human eIF6 Polyclonal Antibody | anti-eIF6 antibody
Rabbit anti-eIF6 Antibody, Affinity Purified
Gene Names
EIF6; CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
eIF6, Antibody; Rabbit anti-eIF6 Antibody, Affinity Purified; eukaryotic translation initiation factor 3A; p27(BBP); p27 beta-4 integrin-binding protein; p27BBP; eukaryotic translation initiation factor 6; EIF3A; CAB; B4 integrin interactor; B(2)GCN homolog; b(2)gcn; ITGB4BP; eIF-6; Eukaryotic translation initiation factor 6; anti-eIF6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-eIF6 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human eIF6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF6 antibody AAA212098 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human eIF6 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF6 antibody AAA212098 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).)
IHC (Immunohiostchemistry)
(Detection of mouse eIF6 by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-eIF6 (Cat. No. AAA212098 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse eIF6 by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-eIF6 (Cat. No. AAA212098 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human eIF6 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF6 (Cat. No. AAA212098 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human eIF6 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF6 (Cat. No. AAA212098 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-eIF6 antibody
Eukaryotic translation initiation factor 6 (eIF6) is insoluble and found both in the nucleus and in the cytoplasm. eIF6 can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.eIF6 binds to the fibronectin type III domains of ITGB4 in hemidesmosomes and may help link ITGB4 to the intermediate filament cytoskeleton [taken from NCBI Entrez Gene (Gene ID: 3692].
Product Categories/Family for anti-eIF6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,599 Da
NCBI Official Full Name
eukaryotic translation initiation factor 6 isoform a
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 6
NCBI Official Symbol
EIF6
NCBI Official Synonym Symbols
CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
NCBI Protein Information
eukaryotic translation initiation factor 6; B4 integrin interactor; p27 beta-4 integrin-binding protein; eukaryotic translation initiation factor 3A
UniProt Protein Name
Eukaryotic translation initiation factor 6
UniProt Gene Name
EIF6
UniProt Entry Name
IF6_HUMAN
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Product Notes
The eIF6 eif6 (Catalog #AAA212098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-eIF6 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eIF6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the eIF6 eif6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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