IHC (Immunohistochemisry)
(Anti-EIF6 antibody, AAA45401, IHC(F)IHC(F): Human Placenta Tissue)
Rabbit integrin beta 4 binding protein Polyclonal Antibody | anti-EIF6 antibody
Anti-integrin beta 4 binding protein antibody
Gene Names
EIF6; CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
integrin beta 4 binding protein, Antibody; Anti-integrin beta 4 binding protein antibody; Eukaryotic translation initiation factor 6; eukaryotic translation initiation factor 6; b(2)gcn antibody; B(2)GCN homolog antibody; B4 integrin interactor antibody; Binding protein of beta-4 integrin antibody; CAB antibody; eIF-6 antibody; EIF3A antibody; EIF6 antibody; Eukaryotic translation initiation factor 3A antibody; Eukaryotic translation initiation factor 6 antibody; IF6_HUMAN antibody; ITGB4BP antibody; OK/SW-cl.27 antibody; p27 beta 4 integrin binding protein antibody; p27(BBP) antibody; p27BBP antibody; RP4-614O4.1 antibody; anti-EIF6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
245
Applicable Applications for anti-EIF6 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human integrin beta 4 binding protein (82-96aa QHIRNSLPDTVQIRR), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti-EIF6 antibody, AAA45401, IHC(F)IHC(F): Human Placenta Tissue)
IHC (Immunohistochemisry)
(Anti-EIF6 antibody, AAA45401, IHC(F)IHC(F): Human Placenta Tissue)
IHC (Immunohiostchemistry)
(Anti-EIF6 antibody, AAA45401, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-EIF6 antibody, AAA45401, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti-EIF6 antibody, AAA45401, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: COLO320 Cell LysateLane 4: SW620 Cell LysateLane 5: HELA Cell LysateLane 6: 293T Cell LysateLane 7: HEPA Cell Lysate)
WB (Western Blot)
(Anti-EIF6 antibody, AAA45401, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: COLO320 Cell LysateLane 4: SW620 Cell LysateLane 5: HELA Cell LysateLane 6: 293T Cell LysateLane 7: HEPA Cell Lysate)
Related Product Information for anti-EIF6 antibody
Description: Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 6(EIF6) detection. Tested with WB, IHC-P, IHC-F in Human, Mouse, Rat.
Background: EIF6(Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al.(1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al.(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.
Background: EIF6(Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al.(1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al.(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al.(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,735 Da
NCBI Official Full Name
Eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132}
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 6
NCBI Official Symbol
EIF6
NCBI Official Synonym Symbols
CAB; EIF3A; eIF-6; p27BBP; ITGB4BP; b(2)gcn; p27(BBP)
NCBI Protein Information
eukaryotic translation initiation factor 6 {ECO:0000255|HAMAP-Rule:MF_03132}; B4 integrin interactor; p27 beta-4 integrin-binding protein; eukaryotic translation initiation factor 3A
UniProt Protein Name
Eukaryotic translation initiation factor 6
UniProt Gene Name
EIF6
UniProt Synonym Gene Names
eIF-6
UniProt Entry Name
IF6_HUMAN
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Product Notes
The EIF6 eif6 (Catalog #AAA45401) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-integrin beta 4 binding protein antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's integrin beta 4 binding protein can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EIF6 eif6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "integrin beta 4 binding protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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