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IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Human Lung cancer TissuePrimary Ab: 20ug/ml Rabbit Anti-Human NE AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human Elastase 2 Polyclonal Antibody | anti-ELA2 antibody
Polyclonal Antibody to Elastase 2, Neutrophil (ELA2)
Gene Names
ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Elastase 2, Antibody; Polyclonal Antibody to Elastase 2, Neutrophil (ELA2); anti-ELA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.5mg/mL (varies by lot)
Applicable Applications for anti-ELA2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant Neutrophil Elastase (Ile30~Gln247) expressed in E.coli (MBS2029953).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Human Lung cancer TissuePrimary Ab: 20ug/ml Rabbit Anti-Human NE AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Human Lung cancer TissuePrimary Ab: 20ug/ml Rabbit Anti-Human NE AntibodyControl: Used PBS instead of primary antibodySecond Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(Vector Red staining on IHC-P;Samples: Human Spleen Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human ELA2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(Vector Red staining on IHC-P;Samples: Human Spleen Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human ELA2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Rectum Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ELA2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Rectum Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ELA2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemisry)
(DAB staining on IHC-P;Samples: Human Lung cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ELA2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemisry)
(DAB staining on IHC-P;Samples: Human Lung cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ELA2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot:Sample: Recombinant ELA2, Human.)
WB (Western Blot)
(Western Blot:Sample: Recombinant ELA2, Human.)
WB (Western Blot)
(Western Blot;Sample: Human Leukocyte lysatePrimary Ab: 2ug/ml Rabbit Anti-Human NE AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot;Sample: Human Leukocyte lysatePrimary Ab: 2ug/ml Rabbit Anti-Human NE AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neutrophil elastase preproprotein
NCBI Official Synonym Full Names
elastase, neutrophil expressed
NCBI Official Symbol
ELANE
NCBI Official Synonym Symbols
GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
NCBI Protein Information
neutrophil elastase
UniProt Protein Name
Neutrophil elastase
UniProt Gene Name
ELANE
UniProt Synonym Gene Names
ELA2; HLE
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Product Notes
The ELA2 elane (Catalog #AAA142535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Elastase 2, Neutrophil (ELA2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Elastase 2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ELA2 elane for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Elastase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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