WB (Western Blot)
(Detection of human and mouse EphA2 by western blot. Samples: Whole cell lysate from A-549 (5, 15, 50 ug), HeLa (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EphA2 antibody AAA211728 (lot AAA211728-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse EphA2 Polyclonal Antibody | anti-EPHA2 antibody
Rabbit anti-EphA2 Antibody, Affinity Purified
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
EphA2, Antibody; Rabbit anti-EphA2 Antibody, Affinity Purified; CTPP1; tyrosine-protein kinase receptor ECK; soluble EPHA2 variant 1; epithelial cell receptor protein tyrosine kinase; Epithelial cell kinase; ephrin type-A receptor 2; CTRCT6; CTPA; ARCC2; ECK; Ephrin type-A receptor 2; EPH receptor A2; anti-EPHA2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-EPHA2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 926 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse EphA2 by western blot. Samples: Whole cell lysate from A-549 (5, 15, 50 ug), HeLa (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EphA2 antibody AAA211728 (lot AAA211728-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human and mouse EphA2 by western blot. Samples: Whole cell lysate from A-549 (5, 15, 50 ug), HeLa (50ug), and mouse NIH 3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EphA2 antibody AAA211728 (lot AAA211728-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human EphA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EphA2 antibody AAA211728 (lot AAA211728-2) used for IP at 6 ug per reaction. EphA2 was also immunoprecipitated by rabbit anti-EphA2 antibody BL8122. For blotting immunoprecipitated EphA2, AAA211728 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human EphA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EphA2 antibody AAA211728 (lot AAA211728-2) used for IP at 6 ug per reaction. EphA2 was also immunoprecipitated by rabbit anti-EphA2 antibody BL8122. For blotting immunoprecipitated EphA2, AAA211728 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-EPHA2 antibody
Ephrin type-A receptor 2 (EphA2) is a member of the Eph family of tyrosine kinase receptors. Eph receptors have been shown to play a critical role in the development of the nervous system, where they regulate patterning during neural development. The ligands for Eph receptors are ephrins. Ephrin receptors and ligands have been found to be frequently overexpressed in a wide variety of cancers. Contrary to the usual finding that the oncogenic properties of tyrosine kinase receptors are promoted by the autophosphorylation of the receptor, it is the dephosphorylation of EphA2 that is associated with its oncogenic activity. The major study of EphA2 has been in relation to its contribution to malignancy and the possibility of targeting it in cancer therapy.
Product Categories/Family for anti-EPHA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,266 Da
NCBI Official Full Name
ephrin type-A receptor 2
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
NCBI Protein Information
ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase
UniProt Protein Name
Ephrin type-A receptor 2
UniProt Gene Name
EPHA2
UniProt Synonym Gene Names
ECK
UniProt Entry Name
EPHA2_HUMAN
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Product Notes
The EPHA2 epha2 (Catalog #AAA211728) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-EphA2 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EphA2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the EPHA2 epha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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