WB (Western Blot)
(Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Rabbit anti-Human, mouse EZH2 Polyclonal Antibody | anti-EZH2 antibody
EZH2 Antibody
WB (Western Blot)
(Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from MDA-MB-468, SW620, T47D cell line, mouse spleen, mouse testis tissue (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Jurkat, T47D cell line (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Jurkat, T47D cell line (from left to right), using EZH2 Antibody. AAA28665 was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HeLa cells using EZH2 Antibody (green) compared to an isotype control of rabbit IgG(blue). AAA28665 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HeLa cells using EZH2 Antibody (green) compared to an isotype control of rabbit IgG(blue). AAA28665 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
WB (Western Blot)
(All lanes : Anti-EZH2 Antibody at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: MDA-MB-231 whole cell lysatesLane 3: MDA-MB-468 whole cell lysatesLane 4: T47D whole cell lysatesLane 5: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-EZH2 Antibody at 1:2000 dilutionLane 1: Jurkat whole cell lysatesLane 2: MDA-MB-231 whole cell lysatesLane 3: MDA-MB-468 whole cell lysatesLane 4: T47D whole cell lysatesLane 5: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-EZH2 Antibody at 1:2000 dilutionLane 1: mouse testis lysatesLane 2: MDA-MB-231 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-EZH2 Antibody at 1:2000 dilutionLane 1: mouse testis lysatesLane 2: MDA-MB-231 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-EZH2 Antibodyat 1:500 dilution + MDA-MB-468 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-EZH2 Antibodyat 1:500 dilution + MDA-MB-468 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-EZH2 Antibodyat 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-EZH2 Antibodyat 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Hiraoka,N., et.al., J. Biol. Chem. 275 (26), 20188-20196 (2000)
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Product Notes
The EZH2 ezh2 (Catalog #AAA28665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EZH2 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EZH2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). WB~~1:1000. Researchers should empirically determine the suitability of the EZH2 ezh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is EZH2 and what does this antibody detect in human and mouse samples?
EZH2 (Enhancer of Zeste Homolog 2) is a histone methyltransferase and a core component of the Polycomb Repressive Complex 2 (PRC2). It plays a vital role in epigenetic gene silencing by adding methyl groups to Histone H3 at Lysine 27. The EZH2 antibody from AAA Biotech is specifically designed to recognize and bind to the EZH2 protein in both human and mouse samples, facilitating accurate detection in comparative biological studies.
Can a Rabbit anti-Human/Mouse EZH2 antibody be used for Western blot?
Yes, this rabbit-derived antibody is highly effective for Western blot (WB) applications. It is designed to recognize the specific epitopes of the EZH2 protein after it has been denatured and separated by electrophoresis. Researchers using AAA Biotech’s EZH2 antibody can expect clear, distinct bands at the predicted molecular weight, allowing for the quantification of protein expression levels across different cell lysates or tissue homogenates from human and mouse origins.
Is EZH2 expression associated with cancer or epigenetic regulation studies?
EZH2 is a central figure in epigenetic regulation, as it mediates the transcriptional repression of various genes. In cancer research, EZH2 is frequently studied because its overexpression is linked to tumor progression, metastasis, and poor prognosis in several malignancies, including breast and prostate cancer. AAA Biotech provides this antibody to support investigators exploring how epigenetic modifications drive oncogenesis and how EZH2 inhibition might serve as a potential therapeutic strategy.
Can this EZH2 antibody be used for immunofluorescence or immunohistochemistry?
This antibody is validated for both immunofluorescence (IF) and immunohistochemistry (IHC). In IF, it allows researchers to visualize the nuclear localization of EZH2 within cultured cells. In IHC, it is used to detect the presence and distribution of EZH2 within formalin-fixed, paraffin-embedded (FFPE) tissue sections. AAA Biotech ensures that the antibody provides the high specificity required to distinguish EZH2 expression patterns in complex cellular environments and varied tissue architectures.
Does EZH2 expression change during cell differentiation or proliferation?
Yes, EZH2 expression is highly dynamic and typically correlates with cell proliferation. It is highly expressed in rapidly dividing cells, such as stem cells and cancer cells, and generally decreases as cells undergo terminal differentiation. By regulating the transition between self-renewal and specialized cell functions, EZH2 acts as a molecular switch. Monitoring these shifts with a reliable antibody is crucial for developmental biology and regenerative medicine research.
Is this antibody suitable for detecting endogenous EZH2 in tissue sections?
The EZH2 antibody is specifically formulated to detect endogenous levels of the protein, meaning it can identify the protein as it naturally occurs within native biological samples. This is particularly important for immunohistochemistry studies where researchers need to observe the actual physiological or pathological expression of EZH2 in human or mouse tissue sections, rather than relying on overexpressed recombinant models, which may not reflect true biological conditions.
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