Rabbit anti-Human FGFR3 Polyclonal Antibody | anti-FGFR3 antibody

FGFR3 Antibody (C-term)

Cat. Num. AAA288484

• Gene Names: FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FGFR3, Antibody; FGFR3 Antibody (C-term); Fibroblast growth factor receptor 3; FGFR-3; CD333; FGFR3; JTK4; anti-FGFR3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FGFR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-806 amino acids from the C-terminal region of human FGFR3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 776-806
• Sequence Length: 791

• Applicable Applications for anti-FGFR3 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.
• Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Endoplasmic reticulum Note=The activated receptor is rapidly internalized and degraded Detected in intracellular vesicles after internalization of the autophosphorylated receptor Isoform 3: Secreted.
• Tissue Location: Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22- week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Isoform 2 is detected in epithelial cells. Isoform 1 is not detected in epithelial cells. Isoform 1 and isoform 2 are detected in fibroblastic cells. FGFR3 is a member of the fibroblast growthfactor receptor family, where amino acidsequence is highly conserved between membersand throughout evolution. FGFR family membersdiffer from one another in their ligand affinitiesand tissue distribution. A full-lengthrepresentative protein would consist of anextracellular region, composed of threeimmunoglobulin-like domains, a singlehydrophobic membrane-spanning segment and acytoplasmic tyrosine kinase domain. Theextracellular portion of the protein interacts withfibroblast growth factors, setting in motion acascade of downstream signals, ultimatelyinfluencing mitogenesis and differentiation. Thisparticular family member binds acidic and basicfibroblast growth hormone and plays a role inbone development and maintenance. Mutationsin this gene lead to craniosynostosis and multipletypes of skeletal dysplasia.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of anti-FGFR3 Pab in Jurkat cell lysate. FGFR3 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-FGFR3 antibody

FGFR3 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.

Product Categories/Family for anti-FGFR3 antibody

Cancer; Cardiovascular; Metabolism

References

Sturla, L.M., et al., Br. J. Cancer 89(7):1276-1284 (2003).
Lievens, P.M., et al., J. Biol. Chem. 278(19):17344-17349 (2003).
Reinhart, E., et al., Mund Kiefer Gesichtschir 7(3):132-137 (2003).
Pehlivan, S., et al., Turk J Pediatr 45(2):99-101 (2003).
Santra, M., et al., Blood 101(6):2374-2376 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4503711
• NCBI GeneID: 2261
• NCBI Accession #: NP_000133.1; NP_001156685.1; NP_075254.1
• NCBI GenBank Nucleotide #: NM_000142.4
• UniProt Accession #: P22607; Q14308; Q16294; Q16608; Q59FL9; D3DVP9; D3DVQ0
• NCBI Official Full Name: fibroblast growth factor receptor 3 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 3
• NCBI Official Symbol: FGFR3
• NCBI Official Synonym Symbols: ACH; CEK2; JTK4; CD333; HSFGFR3EX
• NCBI Protein Information: fibroblast growth factor receptor 3
• UniProt Protein Name: Fibroblast growth factor receptor 3
• UniProt Gene Name: FGFR3
• UniProt Synonym Gene Names: JTK4; FGFR-3
• UniProt Entry Name: FGFR3_HUMAN