IF (Immunofluorescence)
(Immunofluorescence staining of FHIT—310358 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FHIT—310358 polyclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane.)
Rabbit anti-Human FHIT Polyclonal Antibody | anti-FHIT antibody
Anti-FHIT Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
FHIT, Antibody; Anti-FHIT Antibody, Rabbit Polyclonal; FHIT Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-AP3Aase Antibody; Anti-FRA3B Antibody; fragile histidine triad; anti-FHIT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human FHIT
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FHIT antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Conjugation
Unconjugated
Immunogen
E Coli-derived Human FHIT fragment
Preparation
Produced in rabbits immunized with E Coli-derived Human FHIT fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of FHIT—310358 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FHIT—310358 polyclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of FHIT—310358 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human FHIT—310358 polyclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane.)
IHC (Immunohistochemistry)
(Immunochemical staining of human FHIT in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human FHIT in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-FHIT antibody
Fragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically-induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
Product Categories/Family for anti-FHIT antibody
References
Lambert N, et al. (2006) An RNA gene expressed during cortical development evolved rapidly in humans. Nature. 443(7108):167-72. Pekarsky Y, et al. (1998) Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans. Proc Natl Acad Sci. 95(15):8744-9. Ohta M, et al. (1996) The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell. 84(4): 587-97.
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Product Notes
The FHIT (Catalog #AAA258548) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FHIT Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FHIT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the FHIT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FHIT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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