Rabbit FLIP Polyclonal Antibody | anti-CFLAR antibody

FLIP Antibody

Cat. Num. AAA39894

• Gene Names: CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: FLIP Antibody is affinity chromatography purified via peptide column.
• Synonyms: FLIP, Antibody; FLIP Antibody; FLIP; CASH; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1; CASP8 and FADD-like apoptosis regulator; Caspase homolog; anti-CFLAR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FLIP recognizes all FLIP splice variants including FLIPa, b, and y.
• Purity/Purification: FLIP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 480

• Applicable Applications for anti-CFLAR antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: FLIP antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human FLIP. The sequence is identical in all FLIP splice variants.
• Buffer: FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FLIP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of FLIP in mouse liver tissue with FLIP antibody at 20 μg/ml.Red: FLIP Antibody (1159)Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of FLIP in HeLa cells with FLIP antibody at 5 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 20 μg/mL.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of FLIP in (A) HeLa cell and (B) mouse liver tissue lysate with FLIP antibody at 1 μg/ml.)

Related Product Information for anti-CFLAR antibody

FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 2253679
• NCBI GeneID: 8837
• UniProt Accession #: O15519; O14673; O14674; O14675; O15137; O15138; O15356; O15510; O43618; O43619; B4DJE0; B7Z9F9
• Molecular Weight: 44,112 Da
• NCBI Official Full Name: FLICE-like inhibitory protein long form
• NCBI Official Synonym Full Names: CASP8 and FADD-like apoptosis regulator
• NCBI Official Symbol: CFLAR
• NCBI Official Synonym Symbols: CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator; usurpin beta; caspase homolog; inhibitor of FLICE; caspase-eight-related protein; MACH-related inducer of toxicity; FADD-like antiapoptotic molecule 1; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; caspase-like apoptosis regulatory protein
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• UniProt Gene Name: CFLAR
• UniProt Synonym Gene Names: CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_HUMAN