IHC (Immunohiostchemistry)
(Fig: 2 Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using at 1: 2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.)
Rabbit FLIP Polyclonal Antibody | anti-FLIP antibody
Polyclonal Antibody to FLIP
Gene Names
CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
Reactivity
HumanPredicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Serum
Synonyms
FLIP, Antibody; Polyclonal Antibody to FLIP; CASH; CASP8AP1; CLARP; MRIT; Caspase-like apoptosis regulatory protein; Cellular FLICE-like inhibitory protein; FADD-like antiapoptotic molecule 1; Inhibitor of FLICE; Antibody to FLIP; Anti-FLIP Antibody; FLIP Antibody; anti-FLIP antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
480
Applicable Applications for anti-FLIP antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen Information
A synthetic peptideof human FLIP (amino acids 75-92 DRKAVETHLLRNPHLVS) was used as immunogen for this antibody
Antigen
FLIP
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohiostchemistry)
(Fig: 2 Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using at 1: 2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using at 1: 2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.)
IHC (Immunohistochemistry)
(Fig: 1 Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using at 1: 2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-eosin counterstain.)
IHC (Immunohistochemistry)
(Fig: 1 Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using at 1: 2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-eosin counterstain.)
Related Product Information for anti-FLIP antibody
This antibody recognizes FLIP long and short, isoforms that contain the peptide immunogen sequence (DRKAVETHLLRNPHLVSD). Human FLIP is a 480 amino acid protein which contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by death receptors.However, the functions of FLIP remain to be fully elucidated and there is some evidence FLIP may also act as an activator of caspase-8/-10 in certain cases, thereby taking on an apoptosis promoting role.Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the caspase (ICE/CED-3) protease family. Most if not all, forms of apoptosis involve activation of the caspases. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the caspase family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7.
Product Categories/Family for anti-FLIP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
44,112 Da
NCBI Official Full Name
CASP8 and FADD-like apoptosis regulator
NCBI Official Synonym Full Names
CASP8 and FADD like apoptosis regulator
NCBI Official Symbol
CFLAR
NCBI Official Synonym Symbols
CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
NCBI Protein Information
CASP8 and FADD-like apoptosis regulator
UniProt Protein Name
CASP8 and FADD-like apoptosis regulator
UniProt Gene Name
CFLAR
UniProt Synonym Gene Names
CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
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Product Notes
The FLIP cflar (Catalog #AAA78339) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to FLIP reacts with Human Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FLIP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the FLIP cflar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FLIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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