Goat FMR1 / FMRP Polyclonal Antibody | anti-FMR1 antibody

Goat Polyclonal to Human FMR1 / FMRP

Cat. Num. AAA51421

• Gene Names: FMR1; POF; FMRP; POF1; FRAXA
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Hamster, Pig, Rabbit.
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: FMR1 / FMRP, Antibody; Goat Polyclonal to Human FMR1 / FMRP; FMR1, FMRP, POF, Protein FMR-1, Premature ovarian faliure 1, Fragile X mental retardation 1, FRAXA, POF1; anti-FMR1 antibody

• Host: Goat
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Hamster, Pig, Rabbit.
• Clonality: Polyclonal
• Specificity: Human FMR1.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 632

• Applicable Applications for anti-FMR1 antibody: ELISA, WB (Western Blot), IHC (Immunohistochemistry)
• Target: FMR1 / FMRP
• Immunogen: Peptide with sequence C-RTGKDRNQKKEKPD, from the internal region (near the C-Terminus) of the protein sequence according to NP_002015.1.
• Immunogen Species: FMR1 / FMRP antibody was raised againist Human.
• Epitope: aa594-607
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

WB (Western Blot)

(Antibody (0.1 ug/ml) staining of KELLY cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry)

(Anti-FMR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503765
• NCBI GeneID: 2332
• NCBI Accession #: NP_002015.1
• NCBI GenBank Nucleotide #: NM_002024.5
• UniProt Accession #: Q06787; Q16578; Q5PQZ6; Q99054; A6NNH4; D3DWT0; D3DWT1; D3DWT2; G8JL90
• NCBI Official Full Name: fragile X mental retardation protein 1 isoform ISO1
• NCBI Official Synonym Full Names: fragile X mental retardation 1
• NCBI Official Symbol: FMR1
• NCBI Official Synonym Symbols: POF; FMRP; POF1; FRAXA
• NCBI Protein Information: fragile X mental retardation protein 1
• UniProt Protein Name: Fragile X mental retardation protein 1
• UniProt Gene Name: FMR1
• UniProt Synonym Gene Names: FMRP; Protein FMR-1
• UniProt Entry Name: FMR1_HUMAN