Rabbit anti-Human FZD4 Polyclonal Antibody | anti-FZD4 antibody

FZD4 Antibody

Cat. Num. AAA233112

• Gene Names: FZD4; Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, ELISA
• Purity: >95%, Protein G purified
• Synonyms: FZD4, Antibody; FZD4 Antibody; Frizzled-4; Fz-4; hFz4; FzE4; CD344; FZD4; anti-FZD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 537

• Applicable Applications for anti-FZD4 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant Human Frizzled-4 protein (37-222AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-66450 / sc-66451 / sc-66453
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: FZD4 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60 KDaObserved band size: 60 KDa)

IF (Immunofluorescence)

(Immunofluorescent analysis of PC3 cells using AAA233112 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA233112 at dilution of 1:100)

Related Product Information for anti-FZD4 antibody

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 22547161
• NCBI GeneID: 8322
• NCBI Accession #: NP_036325.2
• NCBI GenBank Nucleotide #: NM_012193.3
• UniProt Accession #: Q9ULV1; Q14C97; Q6S9E4; A8K9Q3
• Molecular Weight: 59,881 Da
• NCBI Official Full Name: frizzled-4
• NCBI Official Synonym Full Names: frizzled class receptor 4
• NCBI Official Symbol: FZD4
• NCBI Official Synonym Symbols: Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
• NCBI Protein Information: frizzled-4
• UniProt Protein Name: Frizzled-4
• UniProt Gene Name: FZD4
• UniProt Synonym Gene Names: Fz-4; hFz4
• UniProt Entry Name: FZD4_HUMAN