Rabbit FZD4 / Frizzled 4 Polyclonal Antibody | anti-FZD4 antibody

Rabbit Polyclonal to Human FZD4 / Frizzled 4

Cat. Num. AAA51505

• Gene Names: FZD4; Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Xenopus
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: FZD4 / Frizzled 4, Antibody; Rabbit Polyclonal to Human FZD4 / Frizzled 4; FZD4; CD344; CD344 antigen; EVR1; Frizzled family receptor 4; Frizzled-4; Fz-4; Fz4; HFz4; Exudative vitreoretinopathy 1; FEVR; FZD4S; FzE4; Frizzled 4; Frizzled homolog 4; WNT receptor frizzled-4; anti-FZD4 antibody

• Host: Rabbit
• Reactivity: Bat, Gibbon, Bovine, Chicken, Dog, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat; Predicted Reactivity: Xenopus
• Clonality: Polyclonal
• Specificity: Human FZD4 / Frizzled 4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 537

• Applicable Applications for anti-FZD4 antibody: IHC (Immunohistochemistry)
• Target Species: FZD4 / Frizzled 4
• Immunogen Type: Synthetic peptide
• Immunogen: FZD4 / Frizzled 4 antibody was raised against synthetic 13 amino acid peptide from C-terminus of human FZD4 / Frizzled 4. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Lizard, Xenopus (92%).
• Epitope: C-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

IHC (Immunohistochemisry)

(Anti-FZD4 / Frizzled 4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohiostchemistry)

(Anti-FZD4 / Frizzled 4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry)

(Anti-FZD4 / Frizzled 4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Product Categories/Family for anti-FZD4 antibody

Family: GPCR; Subfamily: Frizzled

NCBI and Uniprot Product Information

• NCBI GI #: 22547161
• NCBI GeneID: 8322
• NCBI Accession #: NP_036325.2
• NCBI GenBank Nucleotide #: NM_012193.3
• UniProt Accession #: Q9ULV1; Q14C97; Q6S9E4; A8K9Q3
• NCBI Official Full Name: frizzled-4
• NCBI Official Synonym Full Names: frizzled class receptor 4
• NCBI Official Symbol: FZD4
• NCBI Official Synonym Symbols: Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
• NCBI Protein Information: frizzled-4; frizzled homolog 4; WNT receptor frizzled-4; frizzled family receptor 4; frizzled 4, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-4
• UniProt Gene Name: FZD4
• UniProt Synonym Gene Names: Fz-4; hFz4
• UniProt Entry Name: FZD4_HUMAN