Rabbit GAP43 Polyclonal Antibody | anti-GAP43 antibody

Phospho-GAP43 (Ser41) Antibody

Cat. Num. AAA329287

• Gene Names: GAP43; B-50; PP46
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: ELISA, Immunocytochemistry, Immunofluorescence
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: GAP43, Antibody; Phospho-GAP43 (Ser41) Antibody; Axonal membrane protein GAP 43; Axonal membrane protein GAP-43; B 50; Calmodulin binding protein P 57; F1; GAP 43; GAP43; Growth Associated Protein 43; Growth-associated protein 43; Nerve Growth Related Peptide; Nerve growth related peptide GAP43; NEUM_HUMAN; Neural phosphoprotein B 50; Neural phosphoprotein B-50; Neuromodulin; Neuron growth associated protein 43; PP46; Protein F1; QtrA-11580; QtrA-13071; anti-GAP43 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-GAP43 (Ser41) Antibody detects endogenous levels of GAP43 only when phosphorylated at Ser41.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GAP43 antibody: ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence)
• Immunogen: A synthesized peptide derived from human GAP43 around the phosphorylation site of Ser41.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated (By similarity). Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2.
• Subunit Structure: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN (By similarity). Interacts (via IQ domain) with calmodulin (By similarity). Binds calmodulin with a greater affinity in the absence of Ca (2+) than in its presence (By similarity).
• Similarity: Belongs to the neuromodulin family.
• Subcellular Location: Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Growth cone membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Synapse. Cell projection>Filopodium membrane>Peripheral membrane protein. Perikaryon. Cell projection>Dendrite. Cell projection>Axon. Cytoplasm.; Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(Staining Hela cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF3715) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

WB (Western Blot)

(Western blot analysis of extracts from COS-7 cells(uv treatment), using Phospho-GAP43 (Ser41) Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-GAP43 antibody

This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.

NCBI and Uniprot Product Information

• NCBI GI #: 194248057
• NCBI GeneID: 2596
• NCBI Accession #: NP_001123536.1
• NCBI GenBank Nucleotide #: NM_001130064.1
• UniProt Accession #: P17677; A8K0Y4
• Molecular Weight: 24,803 Da
• NCBI Official Full Name: neuromodulin isoform 1
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN