Rabbit GFP Polyclonal Antibody | anti-GFP antibody

Anti-GFP Purified

Cat. Num. AAA128933

• Reactivity: Tagged fusion proteins in all species
• Applications: Immunocytochemistry, Western Blot, Immunoprecipitation
• Purity: Purified from Rabbit Serum by Affinity Chromatography
• Synonyms: GFP, Antibody; Anti-GFP Purified; green fluorescent protein; Aquorea victoria green fluorescent protein; EGFP; EYFP; anti-GFP antibody

• Host: Rabbit
• Reactivity: Tagged fusion proteins in all species
• Clonality: Polyclonal
• Clone Number: PAb (476)
• Specificity: The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
• Purity/Purification: Purified from Rabbit Serum by Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-GFP antibody: ICC (Immunocytochemistry), WB (Western Blot), IP (Immunoprecipitation)
• Immunogen: EGFP, a native full-length protein
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for anti-GFP antibody

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (lamda =509 nm) when excited (excitation by Blue or UV light, absorption peak lamda =395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.