GJA1 / CX43 / Connexin 43 Polyclonal Antibody | anti-GJA1 antibody

Goat Polyclonal (IgG) to Rat GJA1 / CX43 / Connexin 43

Cat. Num. AAA52522

• Gene Names: GJA1; HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: GJA1 / CX43 / Connexin 43, Antibody; Goat Polyclonal (IgG) to Rat GJA1 / CX43 / Connexin 43; GJA1 | Connexin-43 | DFNB38 | Connexin 43 | Gap junction alpha-1 protein | GJAL | HSS | Gap junction protein alpha 1 | ODDD | ODD | AVSD3 | CX43 | HLHS1 | ODOD | SDTY3; anti-GJA1 antibody

• Host: GJA1/CX43/Connexin 43 antibody was produced in a Goat
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total connexin 43.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.05% Sodium Azide, 20% Glycerol
• Concentration: 4 mg/ml (varies by lot)
• Sequence Length: 382

• Applicable Applications for anti-GJA1 antibody: IHC (Immunohistochemistry)
• Antigen Type: Recombinant protein
• Epitope: C-terminus
• Target Species: CJA1/CX43/Connexin 43
• Immunogen Species: GJA1/CX43/Connexin 43 antibody was raised against Rat.
• Immunogen: GJA1/CX43/Connexin 43 antibody was raised against purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E.coli.
• Family/Subfamily: Ion Channel / Conexin
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect t this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60 degree C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100 degree C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

WB (Western Blot)

(Western blot. Endogenous Cx43 detected with GJA1 / CX43 / Connexin 43 antibody at 1:1000 dilution. Lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

IF (Immunofluorescence)

(Immunofluorescence. Immunostaining of primary RPE cells with CX43 antibody at 1:100 dilution.)

IHC (Immunohistochemistry)

(Anti-GJA1 / CX43 / Connexin 43 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-GJA1 antibody

Family: Ion Channel; Subfamily: Connexin

NCBI and Uniprot Product Information

• NCBI GI #: 4504001
• NCBI GeneID: 2697
• NCBI Accession #: NP_000156.1
• NCBI GenBank Nucleotide #: NM_000165.4
• UniProt Accession #: P17302; Q6FHU1; Q9Y5I8; B2R5U9
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 1, 43kDa
• NCBI Official Symbol: GJA1
• NCBI Official Synonym Symbols: HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1
• NCBI Protein Information: gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein
• UniProt Protein Name: Gap junction alpha-1 protein
• UniProt Gene Name: GJA1
• UniProt Synonym Gene Names: GJAL; Cx43
• UniProt Entry Name: CXA1_HUMAN