WB (Western Blot)
(Western blot analysis of Connexin 43 expression in C6 (A); HeLa (B); C2C12 (C); mouse heart (D); rat brain (E) whole cell lysates.)
Rabbit GJA1/CX43/Connexin 43 Polyclonal Antibody | anti-GJA1 antibody
Anti-GJA1/CX43/Connexin 43 Antibody (C-Terminus)
Gene Names
GJA1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
Reactivity
HumanPredicted: Chicken, Bovine, Rabbit, Mouse, Dog, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunoaffinity Purified
Synonyms
GJA1/CX43/Connexin 43, Antibody; Anti-GJA1/CX43/Connexin 43 Antibody (C-Terminus); Rabbit Polyclonal to Human GJA1/CX43/Connexin 43; GJA1; Connexin-43; DFNB38; Connexin 43; Gap junction alpha-1 protein; GJAL; HSS; Gap junction protein alpha 1; ODDD; ODD; AVSD3; CX43; HLHS1; ODOD; SDTY3; anti-GJA1 antibody
Host
Rabbit
Reactivity
Human
Predicted: Chicken, Bovine, Rabbit, Mouse, Dog, Rat
Predicted: Chicken, Bovine, Rabbit, Mouse, Dog, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Connexin 43 protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Sequence Length
382
Applicable Applications for anti-GJA1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Connexin 43.
Conjugation
Unconjugated
Epitope
C-Terminus
Preparation and Storage
Store at -20 degree C.
Aliquot to avoid freeze/thaw cycles.
Aliquot to avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of Connexin 43 expression in C6 (A); HeLa (B); C2C12 (C); mouse heart (D); rat brain (E) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of Connexin 43 expression in C6 (A); HeLa (B); C2C12 (C); mouse heart (D); rat brain (E) whole cell lysates.)
IF (Immunofluorescence)
(Immunofluorescent analysis of Connexin 43 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of Connexin 43 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohistochemistry)
(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-GJA1 antibody
Gap junction protein that acts as a regulator of bladder capacity. Agap junction consists of a cluster of closely packed pairs oftransmembrane channels, the connexons, through which materials of low MWdiffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling ofpotassium to the cochlear endolymph.
Product Categories/Family for anti-GJA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,008 Da
NCBI Official Full Name
gap junction alpha-1 protein
NCBI Official Synonym Full Names
gap junction protein alpha 1
NCBI Official Symbol
GJA1
NCBI Official Synonym Symbols
HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA
NCBI Protein Information
gap junction alpha-1 protein
UniProt Protein Name
Gap junction alpha-1 protein
UniProt Gene Name
GJA1
UniProt Synonym Gene Names
GJAL; Cx43
Similar Products
Product Notes
The GJA1 gja1 (Catalog #AAA162374) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GJA1/CX43/Connexin 43 Antibody (C-Terminus) reacts with Human Predicted: Chicken, Bovine, Rabbit, Mouse, Dog, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GJA1/CX43/Connexin 43 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the GJA1 gja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GJA1/CX43/Connexin 43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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