WB (Western Blot)
(Western blot analysis of GLI1 in lysates of PC-3, using GLI1 Antibody.)
Rabbit GLI1 Polyclonal Antibody | anti-GLI1 antibody
GLI1 Antibody
Gene Names
GLI1; GLI; PPD1; PAPA8
Reactivity
Human, Mouse, MonkeyPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
GLI1, Antibody; GLI1 Antibody; Gli 1; GLI; GLI family zinc finger 1; GLI Kruppel family member 1; gli1; GLI1_HUMAN; Glioma associated oncogene 1; Glioma associated oncogene homolog 1 (zinc finger protein); Glioma associated oncogene homolog; Glioma-associated oncogene; Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1; anti-GLI1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
GLI1 Antibody detects endogenous levels of GLI1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1106
Applicable Applications for anti-GLI1 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human GLI1.
Tissue Specificity
Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).
Subcellular Location
Nucleus; Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of GLI1 in lysates of PC-3, using GLI1 Antibody.)
WB (Western Blot)
(Western blot analysis of GLI1 in lysates of PC-3, using GLI1 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from VERO cells, using GLI1 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from VERO cells, using GLI1 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-GLI1 antibody
Description: Acts as a transcriptional activator (PubMed:19706761, PubMed:10806483, PubMed:19878745, PubMed:24311597, PubMed:24217340). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:8378770, PubMed:24217340). May regulate the transcription of specific genes during normal development (PubMed:19706761). May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761). Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable).
Post Translational Modifications: Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.
Subunit Structure: Interacts with KIF7 (By similarity). Interacts with STK36 (PubMed:10806483). Interacts with ZIC1; the interaction enhances transcription activation (PubMed:11238441). Interacts with SUFU; this inhibits transcriptional activation by GLI1 (PubMed:10806483, PubMed:24311597, PubMed:24217340, PubMed:28965847).
Similarity: Belongs to the GLI C2H2-type zinc-finger protein family.
Post Translational Modifications: Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.
Subunit Structure: Interacts with KIF7 (By similarity). Interacts with STK36 (PubMed:10806483). Interacts with ZIC1; the interaction enhances transcription activation (PubMed:11238441). Interacts with SUFU; this inhibits transcriptional activation by GLI1 (PubMed:10806483, PubMed:24311597, PubMed:24217340, PubMed:28965847).
Similarity: Belongs to the GLI C2H2-type zinc-finger protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 119 kDa
Predicted: 118 kDa
Predicted: 118 kDa
NCBI Official Full Name
Zinc finger protein GLI1
NCBI Official Synonym Full Names
GLI family zinc finger 1
NCBI Official Symbol
GLI1
NCBI Official Synonym Symbols
GLI; PPD1; PAPA8
NCBI Protein Information
zinc finger protein GLI1
UniProt Protein Name
Zinc finger protein GLI1
UniProt Gene Name
GLI1
UniProt Synonym Gene Names
GLI
UniProt Entry Name
GLI1_HUMAN
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Product Notes
The GLI1 gli1 (Catalog #AAA328567) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLI1 Antibody reacts with Human, Mouse, Monkey Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's GLI1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the GLI1 gli1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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