WB (Western Blot)
(Anti-Gli3 antibody, AAA44993, Western blottingLane 1: Rat Testis Tissue LysateLane 2: A549 Cell LysateLane 3: SW620 Cell Lysate)
Rabbit Gli3 Polyclonal Antibody | anti-GLI3 antibody
Anti-Gli3 antibody
Gene Names
GLI3; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
Reactivity
Human, RatPredicted to work with: Mouse
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Gli3, Antibody; Anti-Gli3 antibody; Transcriptional activator GLI3; GLI family zinc finger 3; ACLS antibody; DNA binding protein antibody; GCPS antibody; Gli 3 antibody; GLI family zinc finger 3 antibody; GLI Kruppel family member GLI 3 antibody; GLI Kruppel family member GLI3(Greig cephalopolysyndactyly syndrome) antibody; GLI Kruppel family member GLI3 antibody; GLI3 antibody; GLI3 C-terminally truncated form antibody; GLI3 form of 190 kDa antibody; GLI3 form of 83 kDa antibody; GLI3 full length protein antibody; GLI3-190 antibody; GLI3-83 antibody; GLI3_HUMAN antibody; GLI3FL antibody; Glioma associated oncogene family zinc finger 3 antibody; Oncogene GLI3 antibody; PAP A antibody; PAPA 1 antibody; PAPA antibody; PAPA1 antibody; PAPB antibody; PHS antibody; PPD IV antibody; PPDIV antibody; Transcriptional activator GLI3 antibody; Transcriptional repressor GLI3R antibody; Zinc finger protein GLI 3 antibody; Zinc finger protein GLI3 antibody; anti-GLI3 antibody
Host
Rabbit
Reactivity
Human, Rat
Predicted to work with: Mouse
Predicted to work with: Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Sequence Length
1580
Applicable Applications for anti-GLI3 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GLI3 (40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-Gli3 antibody, AAA44993, Western blottingLane 1: Rat Testis Tissue LysateLane 2: A549 Cell LysateLane 3: SW620 Cell Lysate)
WB (Western Blot)
(Anti-Gli3 antibody, AAA44993, Western blottingLane 1: Rat Testis Tissue LysateLane 2: A549 Cell LysateLane 3: SW620 Cell Lysate)
Related Product Information for anti-GLI3 antibody
Description: Rabbit IgG polyclonal antibody for Transcriptional activator GLI3(GLI3) detection. Tested with WB in Human, Mouse, Rat.
Background: GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product(relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus(ci) gene product(Ci), which regulates the Patched(pct), gooseberry(gsb), and decapentaplegic(dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.
Background: GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product(relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus(ci) gene product(Ci), which regulates the Patched(pct), gooseberry(gsb), and decapentaplegic(dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Transcriptional activator GLI3
NCBI Official Synonym Full Names
GLI family zinc finger 3
NCBI Official Symbol
GLI3
NCBI Official Synonym Symbols
PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
NCBI Protein Information
transcriptional activator GLI3; oncogene GLI3; zinc finger protein GLI3; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3
UniProt Protein Name
Transcriptional activator GLI3
UniProt Gene Name
GLI3
UniProt Synonym Gene Names
GLI3-190; GLI3FL; GLI3-83
UniProt Entry Name
GLI3_HUMAN
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Product Notes
The GLI3 gli3 (Catalog #AAA44993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Gli3 antibody reacts with Human, Rat Predicted to work with: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Gli3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the GLI3 gli3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gli3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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