IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GLI3 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
Rabbit anti-Human, Mouse GLI3 Polyclonal Antibody | anti-GLI3 antibody
GLI3 Antibody
Gene Names
GLI3; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
GLI3, Antibody; GLI3 Antibody; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190; anti-GLI3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total GLI3 protein.
Purity/Purification
Antigen affinity purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.7mg/ml (varies by lot)
Sequence Length
1580
Applicable Applications for anti-GLI3 antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Synthetic peptide of human GLI3
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GLI3 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohistochemisry)
(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GLI3 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using GLI3 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using GLI3 Antibody at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
SDS-PAGE
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: Mouse liver tissue, Primary antibody:GLI3 antibody at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
SDS-PAGE
(Gel: 6%SDS-PAGE Lysate: 40 ug, Lane: Mouse liver tissue, Primary antibody:GLI3 antibody at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Related Product Information for anti-GLI3 antibody
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
Product Categories/Family for anti-GLI3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
169,863 Da
NCBI Official Full Name
transcriptional activator GLI3
NCBI Official Synonym Full Names
GLI family zinc finger 3
NCBI Official Symbol
GLI3
NCBI Official Synonym Symbols
PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
NCBI Protein Information
transcriptional activator GLI3
UniProt Protein Name
Transcriptional activator GLI3
UniProt Gene Name
GLI3
UniProt Synonym Gene Names
GLI3-190; GLI3FL; GLI3-83
UniProt Entry Name
GLI3_HUMAN
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Product Notes
The GLI3 gli3 (Catalog #AAA309390) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLI3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLI3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the GLI3 gli3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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