Rabbit anti-Human, Mouse GNL3 Polyclonal Antibody | anti-GNL3 antibody

Rabbit anti-GNL3 Antibody, Affinity Purified

Cat. Num. AAA211045

• Gene Names: GNL3; NS; E2IG3; NNP47; C77032
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: GNL3, Antibody; Rabbit anti-GNL3 Antibody, Affinity Purified; guanine nucleotide-binding protein-like 3; NS; nucleolar GTP-binding protein 3; novel nucleolar protein 47; NNP47; estradiol-induced nucleotide binding protein; E2-induced gene 3 protein; E2IG3; C77032; nucleostemin; guanine nucleotide binding protein-like 3 (nucleolar); Guanine nucleotide-binding protein-like 3; G protein nucleolar 3; anti-GNL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-GNL3 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 499 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody AAA211045 (lot AAA211045-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

WB (Western Blot)

(Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody AAA211045 (lot AAA211045-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human GNL3 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GNL3 antibody AAA211045 (lot AAA211045-3) used for IP at 6 ug per reaction. GNL3 was also immunoprecipitated by a previous lot of this antibody (lot AAA211045-2). For blotting immunoprecipitated GNL3, AAA211045 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry)

(Detection of mouse GNL3 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (Cat. No. AAA211045 lot 3) used at a dilution of 1:200 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (Cat. No. AAA211045 lot 3) used at a dilution of 1:200 (1ug/ml). Detection: DAB)

Related Product Information for anti-GNL3 antibody

GNL3 (guanine nucleotide binding protein-like 3), also known as nucleostemin (NS), was identified as a protein highly expressed in stem cells and downregulated in differentiated cells.GNL3 has also been found to be highly expressed in some cancer cell lines, and may play an important role in the control of cell proliferation. GNL3 functions as a GTPase and functions in the processing of nucleolar pre-rRNA.

Product Categories/Family for anti-GNL3 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 45593130
• NCBI GeneID: 26354
• NCBI Accession #: NP_055181.3; NP_996561.1
• NCBI GenBank Nucleotide #: NM_014366.4
• UniProt Accession #: Q9BVP2; Q5PU80; Q96SV6; Q96SV7; Q9UJY0; B2RDC1
• Molecular Weight: 60,540 Da
• NCBI Official Full Name: guanine nucleotide-binding protein-like 3 isoform 1
• NCBI Official Synonym Full Names: G protein nucleolar 3
• NCBI Official Symbol: GNL3
• NCBI Official Synonym Symbols: NS; E2IG3; NNP47; C77032
• NCBI Protein Information: guanine nucleotide-binding protein-like 3
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• UniProt Gene Name: GNL3
• UniProt Synonym Gene Names: E2IG3; NS
• UniProt Entry Name: GNL3_HUMAN