Rabbit anti-Human Nucleostemin Polyclonal Antibody | anti-GNL3 antibody

Anti-Nucleostemin Antibody, Rabbit Polyclonal

Cat. Num. AAA254311

• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: Nucleostemin, Antibody; Anti-Nucleostemin Antibody, Rabbit Polyclonal; GNL3/Nucleostemin Antibody, Rabbit PAb, Antigen Affinity Purified; guanine nucleotide binding protein-like 3 (nucleolar); Anti-C77032 Antibody; Anti-E2IG3 Antibody; Anti-NNP47 Antibody; Anti-NS Antibody; anti-GNL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GNL3/Nucleostemin
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GNL3 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human GNL3/Nucleostemin
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GNL3/Nucleostemin, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-GNL3 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.))

IF (Immunofluorescence)

(Immunofluorescence staining of GNL3 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GNL3 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus..)

Related Product Information for anti-GNL3 antibody

GNL3 (G Protein Nucleolar 3, also known as NS) is a Protein Coding gene. GNL3 belongs to the TRAFAC class YlqF/YawG GTPase family. The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. GNL3 is widely expressed in the urinary bladder, prostate, and other tissues. GNL3 protein has been recently documented to be a nucleolar protein that was abundantly expressed in stem cells and cancer cells. NS may contribute to sorafenib resistance in HCC cells through the modulation of the p53 pathway and Bcl-2 proteins. That the combination of silencing NS expression and sorafenib treatment is a promising therapeutic strategy in the treatment of HCC. GNL3 is a potential genetic modifier for knee osteoarthritis (KOA) development.

NCBI and Uniprot Product Information

• UniProt Accession #: Q9BVP2
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• UniProt Gene Name: GNL3
• UniProt Synonym Gene Names: E2IG3; NS
• UniProt Entry Name: GNL3_HUMAN