WB (Western Blot)
(Dilution: Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-GPR177(bs-10196R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62 kDObserved band size: 51 kD)
Rabbit GPR177 Polyclonal Antibody | anti-GPR177 antibody
GPR177 Polyclonal Antibody
Gene Names
WLS; EVI; MRP; GPR177; mig-14; C1orf139
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GPR177, Antibody; GPR177 Polyclonal Antibody; Protein wntless homolog; Integral membrane protein GPR177; Protein evenness interrupted homolog; EVI; Putative NF-kappa-B-activating protein 373; WLS; C1orf139; GPR177; anti-GPR177 antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-GPR177 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Dilution: Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-GPR177(bs-10196R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62 kDObserved band size: 51 kD)
WB (Western Blot)
(Dilution: Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-GPR177(bs-10196R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 62 kDObserved band size: 51 kD)
IHC (Immunohiostchemistry)
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-GPR177 Polyclonal Antibody, Unconjugated(bs-15388R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohiostchemistry)
(Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-GPR177 Polyclonal Antibody, Unconjugated(bs-15388R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IF (Immunofluorescence)
(Tissue/cell: rat kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-GPR177 Polyclonal Antibody, Unconjugated(bs-15388R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
IF (Immunofluorescence)
(Tissue/cell: rat kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min;Incubation: Anti-GPR177 Polyclonal Antibody, Unconjugated(bs-15388R) 1:200, overnight at 4 degree C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37 degree C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,571 Da
NCBI Official Full Name
protein wntless homolog isoform 2
NCBI Official Synonym Full Names
wntless Wnt ligand secretion mediator
NCBI Official Symbol
WLS
NCBI Official Synonym Symbols
EVI; MRP; GPR177; mig-14; C1orf139
NCBI Protein Information
protein wntless homolog; G protein-coupled receptor 177; integral membrane protein GPR177; protein evenness interrupted homolog; putative NF-kappa-B-activating protein 373; putative NFkB activating protein 373
UniProt Protein Name
Protein wntless homolog
UniProt Gene Name
WLS
UniProt Synonym Gene Names
C1orf139; GPR177; EVI
UniProt Entry Name
WLS_HUMAN
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Product Notes
The GPR177 wls (Catalog #AAA291621) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR177 Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's GPR177 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the GPR177 wls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR177, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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