Rabbit GST Polyclonal Antibody | anti-GST antibody

Anti-GST Antibody, Rabbit Polyclonal

Cat. Num. AAA259426

• Reactivity: Schistosoma japonicum
• Applications: Immunocytochemistry, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: GST, Antibody; Anti-GST Antibody, Rabbit Polyclonal; GST Antibody, Rabbit PAb, Antigen Affinity Purified; anti-GST antibody

• Host: Rabbit
• Reactivity: Schistosoma japonicum
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Schistosoma japonicum GST
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GST antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Recombinant Schistosoma japonicum GST Protein ( P08515; Met1-Lys218). GST specific IgG was purified by Schistosoma japonicum GST affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

IF (Immunofluorescence)

(Immunofluorescence staining of GST in HCT116 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human GST polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

WB (Western Blot)

(Anti-GST-Tag rabbit polyclonal antibody at 1:500Lane A: GST-ARG1-DYKDDDDK transfected 293 Cell Lysate(30ug)Lane B: DYKDDDDK-AGR1-GST transfected 293 Cell Lysate(30ug)Lane C: Negative control(30ug)SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique. Performed under reducing conditions.)

Related Product Information for anti-GST antibody

Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 22amino acids(roughly 26 KDa), which, compared to other tags like the Myc- or the DYKDDDDK-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification.

References

Dougla. KT. e. al.. 1987. Ad. Enzymo. Rela. Area. Mo. Biol.59. 103. 67.Becket. GJ. e. al.. 1993. Ad. Cli. Chem. 30. 281-380. Wilc. MC. e. al.. 1994. Biochi. Biophy. Acta. 1205(1). 1-18. Leave. MJ. e. al.. 1998. Marin. Environmenta. Research. 46(1-5). 71-4.

NCBI and Uniprot Product Information

• NCBI GI #: 121697
• UniProt Accession #: P08515
• Molecular Weight: 25.4 kDa (218aa)
• NCBI Official Full Name: Glutathione S-transferase class-mu 26 kDa isozyme
• UniProt Protein Name: Glutathione S-transferase class-mu 26 kDa isozyme
• UniProt Gene Name: GST 26
• UniProt Synonym Gene Names: GST 26
• UniProt Entry Name: GST26_SCHJA