IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit HARS Polyclonal Antibody | anti-HARS antibody
HARS Antibody (N-term)
Gene Names
HARS; HRS; USH3B
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HARS, Antibody; HARS Antibody (N-term); Histidine--tRNA ligase; cytoplasmic; Histidyl-tRNA synthetase; HisRS; HARS; HRS; anti-HARS antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human HARS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
49-78
Sequence Length
489
Applicable Applications for anti-HARS antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HARS antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-HARS Pab in K562 cell line lysates (35ug/lane).HARS (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-HARS Pab in K562 cell line lysates (35ug/lane).HARS (arrow) was detected using the purified Pab.)
Related Product Information for anti-HARS antibody
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Product Categories/Family for anti-HARS antibody
References
Levine,S.M., Arthritis Rheum. 56 (8), 2729-2739 (2007)
Lu,Q., Proc. Natl. Acad. Sci. U.S.A. 100 (13), 7626-7631 (2003)
Lu,Q., Proc. Natl. Acad. Sci. U.S.A. 100 (13), 7626-7631 (2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57411
NCBI Official Full Name
histidine--tRNA ligase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
histidyl-tRNA synthetase
NCBI Official Symbol
HARS
NCBI Official Synonym Symbols
HRS; USH3B
NCBI Protein Information
histidine--tRNA ligase, cytoplasmic
UniProt Protein Name
Histidine--tRNA ligase, cytoplasmic
UniProt Gene Name
HARS
UniProt Synonym Gene Names
HRS; HisRS
UniProt Entry Name
SYHC_HUMAN
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Product Notes
The HARS hars (Catalog #AAA289352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 49-78. The HARS Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's HARS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HARS hars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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