IHC (Immunohiostchemistry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Rabbit Histidyl tRNA Synthetase Polyclonal Antibody | anti-HARS antibody
Polyclonal Antibody to Histidyl tRNA Synthetase (HARS)
Gene Names
HARS; HRS; CMT2W; USH3B
Reactivity
Human, Mouse, Rat, Pig
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Histidyl tRNA Synthetase, Antibody; Polyclonal Antibody to Histidyl tRNA Synthetase (HARS); HRS; Jo1; Jo-1; HisRS; Histidine tRNA Ligase 1,Cytoplasmic; anti-HARS antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HARS. It has been selected for its ability to recognize HARS in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
514
Applicable Applications for anti-HARS antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Fragment
HARS (Met1~Gln294)
Conjugate
No Conjugate
Cross Reactivity
Human
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant HARS (Met1~Gln294) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
IHC (Immunohiostchemistry)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,847 Da
NCBI Official Full Name
histidine--tRNA ligase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
histidyl-tRNA synthetase
NCBI Official Symbol
HARS
NCBI Official Synonym Symbols
HRS; CMT2W; USH3B
NCBI Protein Information
histidine--tRNA ligase, cytoplasmic
UniProt Protein Name
Histidine--tRNA ligase, cytoplasmic
UniProt Gene Name
HARS
UniProt Synonym Gene Names
HRS; HisRS
Similar Products
Product Notes
The HARS hars (Catalog #AAA145746) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Histidyl tRNA Synthetase (HARS) reacts with Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Histidyl tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HARS hars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histidyl tRNA Synthetase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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