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IP (Immunoprecipitation)
(Immunoprecipitating HARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119700 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119700(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Rabbit anti-Human Histidine-tRNA ligase Polyclonal Antibody | anti-HARS antibody
Rabbit anti-human Histidine-tRNA ligase, cytoplasmic polyclonal Antibody(HARS)
Gene Names
HARS; HRS; USH3B
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
Histidine-tRNA ligase, Antibody; Rabbit anti-human Histidine-tRNA ligase, cytoplasmic polyclonal Antibody(HARS); Histidyl-tRNA synthetase; HisRS; HARS; HRS; anti-HARS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
469
Applicable Applications for anti-HARS antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human Histidine--tRNA ligase, cytoplasmic protein (1-180aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-26881 / sc-26883
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
IP (Immunoprecipitation)
(Immunoprecipitating HARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119700 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119700(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating HARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of AAA119700 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: AAA119700(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human liver cancer using AAA119700 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human liver cancer using AAA119700 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human rectum using AAA119700 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human rectum using AAA119700 at dilution 1:100)
WB (Western Blot)
(All lanes: Histidine--tRNA ligase, cytoplasmic antibody at 6ug/mlLane 1:Hela whole cell lysateLane 2:K562 whole cell lysateLane 3:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 58,53,51,55 kDaObserved band size: 57 kDa)
WB (Western Blot)
(All lanes: Histidine--tRNA ligase, cytoplasmic antibody at 6ug/mlLane 1:Hela whole cell lysateLane 2:K562 whole cell lysateLane 3:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 58,53,51,55 kDaObserved band size: 57 kDa)
Related Product Information for anti-HARS antibody
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,847 Da
NCBI Official Full Name
histidine--tRNA ligase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
histidyl-tRNA synthetase
NCBI Official Symbol
HARS
NCBI Official Synonym Symbols
HRS; USH3B
NCBI Protein Information
histidine--tRNA ligase, cytoplasmic
UniProt Protein Name
Histidine--tRNA ligase, cytoplasmic
UniProt Gene Name
HARS
UniProt Synonym Gene Names
HRS; HisRS
UniProt Entry Name
SYHC_HUMAN
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Product Notes
The HARS hars (Catalog #AAA119700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Histidine-tRNA ligase, cytoplasmic polyclonal Antibody(HARS) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histidine-tRNA ligase can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HARS hars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histidine-tRNA ligase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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