IHC (Immunohistochemisry)
(HCLS1 Antibody (C-term) (AAA284800)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HCLS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human HCLS1 Polyclonal Antibody | anti-HCLS1 antibody
HCLS1 Antibody (C-term)
Gene Names
HCLS1; HS1; p75; CTTNL; lckBP1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HCLS1, Antibody; HCLS1 Antibody (C-term); Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; LckBP1; p75; HCLS1; HS1; anti-HCLS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HCLS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-391 amino acids from the C-terminal region of human HCLS1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
362-391
Sequence Length
209
Applicable Applications for anti-HCLS1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(HCLS1 Antibody (C-term) (AAA284800)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HCLS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(HCLS1 Antibody (C-term) (AAA284800)immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HCLS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(HCLS1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HCLS1 antibody detected the HCLS1 protein (arrow).)
WB (Western Blot)
(HCLS1 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HCLS1 antibody detected the HCLS1 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from K562, Raji cell line (from left to right), using HCLS1 Antibody (C-term). AAA284800 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from K562, Raji cell line (from left to right), using HCLS1 Antibody (C-term). AAA284800 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-HCLS1 antibody
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Product Categories/Family for anti-HCLS1 antibody
References
Muzio, M., et al. Leukemia 21(9):2067-2070(2007)
Lim, J., et al. Cell 125(4):801-814(2006)
Hao, J.J., et al. J. Biol. Chem. 280(45):37988-37994(2005)
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)
Brunati, A.M., et al. J. Biol. Chem. 280(22):21029-21035(2005)
Lim, J., et al. Cell 125(4):801-814(2006)
Hao, J.J., et al. J. Biol. Chem. 280(45):37988-37994(2005)
Tao, W.A., et al. Nat. Methods 2(8):591-598(2005)
Brunati, A.M., et al. J. Biol. Chem. 280(22):21029-21035(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54014
NCBI Official Full Name
hematopoietic lineage cell-specific protein isoform 1
NCBI Official Synonym Full Names
hematopoietic cell-specific Lyn substrate 1
NCBI Official Symbol
HCLS1
NCBI Official Synonym Symbols
HS1; p75; CTTNL; lckBP1
NCBI Protein Information
hematopoietic lineage cell-specific protein
UniProt Protein Name
Hematopoietic lineage cell-specific protein
UniProt Gene Name
HCLS1
UniProt Synonym Gene Names
HS1
UniProt Entry Name
HCLS1_HUMAN
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Product Notes
The HCLS1 hcls1 (Catalog #AAA284800) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 362-391. The HCLS1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HCLS1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HCLS1 hcls1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCLS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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