Rabbit HCLS1 Polyclonal Antibody | anti-HCLS1 antibody

HCLS1 Antibody

Cat. Num. AAA326645

• Gene Names: HCLS1; HS1; p75; CTTNL; lckBP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientfic).
• Synonyms: HCLS1, Antibody; HCLS1 Antibody; Cortactin like; CTTNL; HCLS 1; Hcls1; HCLS1_HUMAN; Hematopoietic cell specific Lyn substrate 1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS 1; HS1; LckBP1; OTTHUMP00000215180; OTTHUMP00000215182; p75; anti-HCLS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HCLS1 antibody detects endogenous levels of total HCLS1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientfic).
• Form/Format: Liquid; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 449

• Applicable Applications for anti-HCLS1 antibody: ELISA, IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human HCLS1, corresponding to a region within the internal amino acids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(AAA326645 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot)

(Western blot analysis of extracts of HEK-293, using HCLS1 antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-HCLS1 antibody

Description: HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1, 2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCgamma1 (6). HS1 also plays an important role in platelet activation (7).
Function: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Subunit Structure: Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS (By similarity). Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54 (By similarity).
Post-translational Modifications: Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES.

NCBI and Uniprot Product Information

• NCBI GI #: 635574580
• NCBI GeneID: 3059
• NCBI Accession #: NP_001278970.1
• NCBI GenBank Nucleotide #: NM_001292041.1
• UniProt Accession #: P14317; Q53Y93; Q6IBK9; Q9UDK0; B4DQ69
• Molecular Weight: 54 kDa
• NCBI Official Full Name: hematopoietic lineage cell-specific protein isoform 2
• NCBI Official Synonym Full Names: hematopoietic cell-specific Lyn substrate 1
• NCBI Official Symbol: HCLS1
• NCBI Official Synonym Symbols: HS1; p75; CTTNL; lckBP1
• NCBI Protein Information: hematopoietic lineage cell-specific protein
• UniProt Protein Name: Hematopoietic lineage cell-specific protein
• UniProt Gene Name: HCLS1
• UniProt Synonym Gene Names: HS1