IHC (Immunohistochemistry)
(Primary Antibody: AAA75866; 1:100 dilution in IHC Blocking Buffer. Dab (brown)staining and Hematoxylin QS (blue) counterstain. 20X magnification on Leica DM4000B , FPPE section. Dilutions are for references only. Applications not listed are not necessarily preduced from working with this antibody. Investigators intending to use an application that has not been verified can request a complimantary sample.)
Rabbit HE4 Polyclonal Antibody | anti-HE4 antibody
HE4 Antibody
Gene Names
WFDC2; HE4; WAP5; EDDM4; dJ461P17.6
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Inmobilized antigen affinity chromatography.
Synonyms
HE4, Antibody; HE4 Antibody; EDDM4; epididymal protein 4; Epididymal secretory protein E4; Epididymis specific whey acidic protein type four disulfide core; HE 4; Major epididymis specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4; WAP four disulfide core domain 2; WAP5; WFDC2 antibody; anti-HE4 antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Inmobilized antigen affinity chromatography.
Form/Format
Affinity Purified Immunoglobulins
Concentration
100 ug antibody in 150-170ul antibody stabilization buffer. (varies by lot)
Applicable Applications for anti-HE4 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Immunogen
Synthetic peptide taken within amino acid region 70-120 on human WAP four-disulfide core domain protein 2.
Expression
male reproductive system; regions of the respiratory tract; nasopharynx; number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines.Initially described as being exclusively transcribed in the epididymis
Determinant
C-epitope
Molecular Function
Aspartic protease inhibitor, Protease inhibitor, Serine protease inhibitor, Thiol protease inhibitor
Structure
Homotrimer; disulfide-linked
Subcellular Location
Extracellular space; Extracellular vesicular exosome; Secreted
Preparation and Storage
Store at -20°C for long term storage.
IHC (Immunohistochemistry)
(Primary Antibody: AAA75866; 1:100 dilution in IHC Blocking Buffer. Dab (brown)staining and Hematoxylin QS (blue) counterstain. 20X magnification on Leica DM4000B , FPPE section. Dilutions are for references only. Applications not listed are not necessarily preduced from working with this antibody. Investigators intending to use an application that has not been verified can request a complimantary sample.)
IHC (Immunohistochemistry)
(Primary Antibody: AAA75866; 1:100 dilution in IHC Blocking Buffer. Dab (brown)staining and Hematoxylin QS (blue) counterstain. 20X magnification on Leica DM4000B , FPPE section. Dilutions are for references only. Applications not listed are not necessarily preduced from working with this antibody. Investigators intending to use an application that has not been verified can request a complimantary sample.)
Related Product Information for anti-HE4 antibody
Description: WAP domain contains protein HE4-V3 Antibody Affinity Purified C-epitope.
Overview: The whey acidic protein (WAP) domain is a conserved motif, containing eight cysteines found in a characteristic 4-disulphide core arrangement, that is present in a number of otherwise unrelated proteins. One of these proteins, which contains two WAP domains, is HE4 (also known as WFDC2). HE4 was originally described as an epididymis specific protein, but more recently suggested to be a putative serum tumor marker for ovarian cancer and a presumptive role in natural immunity. The HE4 protein expression is not only confined to epididymis but is expressed in a number of normal human tissues out side the reproductive system, including regions of the respiratory tract and nasopharynx and in a subset of lung tumor cell lines. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituitary gland, thyroid, and kidney. Highest level of expression of ESPE4 was observed in adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas (1). The WFDC2 gene undergoes extensive splicing in malignant tissues that gives rise to five WAP domain containing iso forms (2).
WFDC2 is expressed in some epithelial cells of the upper airways as well as in mucous cells and ducts of sub mucosal glands. No staining was seen in the peripheral lung tissue. Intense staining is found in major salivary glands and in minor glands of the nose, sinuses, posterior tongue and tonsil. Studies with the related protein Secretory Leukocyte Protease Inhibitor (SLPI) show that although both proteins are expressed in similar tissues, the precise cellular localization differs. Significant increases in expression and localization of WFDC2 are seen in patients with Cystic Fibrosis (3). ESPE2 protein is a 124 amino acids (15 kDa) protein. ESPE4 protein has sequence homology to extracellular proteinase inhibitor and is localized on human chromosome 20. The ESPE4 protein is a secreted glycoprotein (Asn-44) that is over expressed in serous and endometrioid ovarian carcinomas (4). There are at least 5 named variants (HE4-V1 to HE4-V5) expressed as a result of alternate splicing and they serve as endopeptidase inhibitors.
The HE4-selective antibodies were generated against a synthetic peptide taken within amino acid region 70-120 on human WAP fourdisulfide core domain protein 2. The HE4 antibodies were affinity purified over immobilized antigen based affinity chromatography. The purified immunoglobulins are stabilized in antibody stabilization buffer. Antigenic blocking peptide (P-HE4.2) and western blot positive controls (PC-HE4) are available.
Overview: The whey acidic protein (WAP) domain is a conserved motif, containing eight cysteines found in a characteristic 4-disulphide core arrangement, that is present in a number of otherwise unrelated proteins. One of these proteins, which contains two WAP domains, is HE4 (also known as WFDC2). HE4 was originally described as an epididymis specific protein, but more recently suggested to be a putative serum tumor marker for ovarian cancer and a presumptive role in natural immunity. The HE4 protein expression is not only confined to epididymis but is expressed in a number of normal human tissues out side the reproductive system, including regions of the respiratory tract and nasopharynx and in a subset of lung tumor cell lines. HE4 gene expression was highest in normal human trachea and salivary gland, and to a lesser extent, lung, prostate, pituitary gland, thyroid, and kidney. Highest level of expression of ESPE4 was observed in adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas (1). The WFDC2 gene undergoes extensive splicing in malignant tissues that gives rise to five WAP domain containing iso forms (2).
WFDC2 is expressed in some epithelial cells of the upper airways as well as in mucous cells and ducts of sub mucosal glands. No staining was seen in the peripheral lung tissue. Intense staining is found in major salivary glands and in minor glands of the nose, sinuses, posterior tongue and tonsil. Studies with the related protein Secretory Leukocyte Protease Inhibitor (SLPI) show that although both proteins are expressed in similar tissues, the precise cellular localization differs. Significant increases in expression and localization of WFDC2 are seen in patients with Cystic Fibrosis (3). ESPE2 protein is a 124 amino acids (15 kDa) protein. ESPE4 protein has sequence homology to extracellular proteinase inhibitor and is localized on human chromosome 20. The ESPE4 protein is a secreted glycoprotein (Asn-44) that is over expressed in serous and endometrioid ovarian carcinomas (4). There are at least 5 named variants (HE4-V1 to HE4-V5) expressed as a result of alternate splicing and they serve as endopeptidase inhibitors.
The HE4-selective antibodies were generated against a synthetic peptide taken within amino acid region 70-120 on human WAP fourdisulfide core domain protein 2. The HE4 antibodies were affinity purified over immobilized antigen based affinity chromatography. The purified immunoglobulins are stabilized in antibody stabilization buffer. Antigenic blocking peptide (P-HE4.2) and western blot positive controls (PC-HE4) are available.
Product Categories/Family for anti-HE4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
WAP four-disulfide core domain protein 2
NCBI Official Synonym Full Names
WAP four-disulfide core domain 2
NCBI Official Symbol
WFDC2
NCBI Official Synonym Symbols
HE4; WAP5; EDDM4; dJ461P17.6
NCBI Protein Information
WAP four-disulfide core domain protein 2
UniProt Protein Name
WAP four-disulfide core domain protein 2
UniProt Gene Name
WFDC2
UniProt Synonym Gene Names
HE4; WAP5
UniProt Entry Name
WFDC2_HUMAN
Similar Products
Product Notes
The HE4 wfdc2 (Catalog #AAA75866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HE4 Antibody reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HE4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the HE4 wfdc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HE4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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