WB (Western Blot)
(Detection of human HERC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC1 antibody AAA211673 (lot AAA211673-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human HERC1 Polyclonal Antibody | anti-HERC1 antibody
Rabbit anti-HERC1 Antibody, Affinity Purified
Gene Names
HERC1; p532; p619; MDFPMR
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
HERC1, Antibody; Rabbit anti-HERC1 Antibody, Affinity Purified; HECT domain and RCC1-like domain-containing protein 1; probable E3 ubiquitin-protein ligase HERC1; p619; p532; HECT-type E3 ubiquitin transferase HERC1; hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 1; guanine nucleotide exchange factor p532; MDFPMR; Probable E3 ubiquitin-protein ligase HERC1; HECT and RLD domain containing E3 ubiquitin protein ligase family member 1; anti-HERC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-HERC1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 2400 and 2450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HERC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC1 antibody AAA211673 (lot AAA211673-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human HERC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC1 antibody AAA211673 (lot AAA211673-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human HERC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC1 antibody AAA211673 (lot AAA211673-2) used for IP at 3 ug per reaction. HERC1 was also immunoprecipitated by rabbit anti-HERC1 antibody For blotting immunoprecipitated HERC1, AAA211673 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human HERC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC1 antibody AAA211673 (lot AAA211673-2) used for IP at 3 ug per reaction. HERC1 was also immunoprecipitated by rabbit anti-HERC1 antibody For blotting immunoprecipitated HERC1, AAA211673 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-HERC1 antibody
HERC1 (HECT domain and RCC1-like domain-containing protein 1) belongs to the HERC family of ubiquitin ligases. It is involved in membrane traffic via its ability to bind clathrin and its guanine nucleotide exchange factor (GEF) activity for Arf and Rab. HERC1 has been found to interact with TSC2 (tuberous sclerosis complex 2) and may be involved with its destabilization.
Product Categories/Family for anti-HERC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
532,228 Da
NCBI Official Full Name
probable E3 ubiquitin-protein ligase HERC1
NCBI Official Synonym Full Names
HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
NCBI Official Symbol
HERC1
NCBI Official Synonym Symbols
p532; p619; MDFPMR
NCBI Protein Information
probable E3 ubiquitin-protein ligase HERC1
UniProt Protein Name
Probable E3 ubiquitin-protein ligase HERC1
UniProt Gene Name
HERC1
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Product Notes
The HERC1 herc1 (Catalog #AAA211673) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HERC1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HERC1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HERC1 herc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HERC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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