WB (Western Blot)
(HIF-1 alpha antibody (pAb) tested by Western blot. Detection of HIF-1 alpha by Western blot analysis with 20 ug COS-7 nuclear extract either untreated (lane 1) or treated with CoCl2 (lane 2).)
Rabbit anti-Human HIF-1 alpha Polyclonal Antibody | anti-HIF-1alpha antibody
HIF-1 alpha antibody (pAb)
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Synonyms
HIF-1 alpha, Antibody; HIF-1 alpha antibody (pAb); HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; anti-HIF-1alpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-HIF-1alpha antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This HIF-1 alpha antibody was raised against a synthetic peptide corresponding to the C-terminal region of human HIF-1 alpha.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(HIF-1 alpha antibody (pAb) tested by Western blot. Detection of HIF-1 alpha by Western blot analysis with 20 ug COS-7 nuclear extract either untreated (lane 1) or treated with CoCl2 (lane 2).)
WB (Western Blot)
(HIF-1 alpha antibody (pAb) tested by Western blot. Detection of HIF-1 alpha by Western blot analysis with 20 ug COS-7 nuclear extract either untreated (lane 1) or treated with CoCl2 (lane 2).)
ChIP (Chromatin Immunoprecipitation)
(HIF-1 alpha antibody (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(HIF-1 alpha antibody (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(HIF-1 alpha antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Hif-1 alpha binding sites across the genome. The images show binding in the promoters of Hif1a target genes that regulate metabolism.)
ChIP (Chromatin Immunoprecipitation)
(HIF-1 alpha antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Hif-1 alpha binding sites across the genome. The images show binding in the promoters of Hif1a target genes that regulate metabolism.)
Related Product Information for anti-HIF-1alpha antibody
Short Description: Our HIF-1 alpha antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human samples.
Background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor involved in the regulation of oxygen homeostasis. It regulates the physiological responses to low oxygen levels (hypoxia) and the pathophysiology of heart attack, cancer, stroke and chronic lung disease. HIF-1 is a heterodimeric protein that consists of two subunits, HIF-1 alpha and HIF-1 beta (also known as ARNT1, the aryl hydrocarbon nuclear translocator). While HIF-1 beta is constitutively expressed, the expression of HIF-1 alpha is induced by oxygen concentrations below 6%. Heterodimerization is required to bind the hypoxia response element (HRE).
Background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor involved in the regulation of oxygen homeostasis. It regulates the physiological responses to low oxygen levels (hypoxia) and the pathophysiology of heart attack, cancer, stroke and chronic lung disease. HIF-1 is a heterodimeric protein that consists of two subunits, HIF-1 alpha and HIF-1 beta (also known as ARNT1, the aryl hydrocarbon nuclear translocator). While HIF-1 beta is constitutively expressed, the expression of HIF-1 alpha is induced by oxygen concentrations below 6%. Heterodimerization is required to bind the hypoxia response element (HRE).
Product Categories/Family for anti-HIF-1alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32.8kDa (298aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1 alpha subunit
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
NCBI Protein Information
hypoxia-inducible factor 1-alpha
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The HIF-1alpha hif1a (Catalog #AAA59909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF-1 alpha antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIF-1 alpha can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the HIF-1alpha hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF-1 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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