WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.)
Rabbit HIF1A Polyclonal Antibody | anti-HIF1A antibody
HIF1A antibody - N-terminal region
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Tested Species Reactivity: HumanPredicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Synonyms
HIF1A, Antibody; HIF1A antibody - N-terminal region; anti-HIF1A antibody
Host
Rabbit
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/ml (varies by lot)
Sequence
EGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVS
Applicable Applications for anti-HIF1A antibody
IHC (Immunohistochemistry), WB (Western Blot)
Protein Size (# AA)
826 amino acids
Blocking Peptide
For anti-HIF1A (MBS3200251) antibody is
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human HIF1A
Enhanced Validation
WB
Y
SPR
YCHAROS
Y
SPR
YCHAROS
Protein Interactions
HDAC1; EP300; ARNTL; HDAC3; VHL; UBC; SUMO3; EGLN3; HIF1AN; SENP3; HSP90AA1; ATM; ARNT2; SART3; SUMO2; SIN3A; TRIM28; HSPA4; TRAF6; GNB2L1; EGLN1; SQSTM1; SUMO1; HEXIM1; LAMP2; HSPA8; RB1; SP1; SMAD3; MAFK; PCGF2; TCEB2; TCEB1; MAFG; HSP90AB1; DCUN1D1; RB
Predicted Homology
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 87%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 86%
Replacement Item
This antibody may replace item sc-10790 from Santa Cruz Biotechnology.
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.)
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The protein may be modified by phosphorylation.)
IHC (Immunohiostchemistry)
(Rabbit Anti-HIF1A antibodyParaffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)
IHC (Immunohiostchemistry)
(Rabbit Anti-HIF1A antibodyParaffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)
Application Data
(Rabbit Anti-HIF1A AntibodyCatalog Number: AAA197109Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow TissueObserved Staining: Nucleus, CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Application Data
(Rabbit Anti-HIF1A AntibodyCatalog Number: AAA197109Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow TissueObserved Staining: Nucleus, CytoplasmPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)
Related Product Information for anti-HIF1A antibody
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF1A is the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
Product Categories/Family for anti-HIF1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93 kDa
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 2
NCBI Official Synonym Full Names
hypoxia inducible factor 1 subunit alpha
NCBI Official Symbol
HIF1A
NCBI Official Synonym Symbols
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
NCBI Protein Information
hypoxia-inducible factor 1-alpha
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
HIF1A
UniProt Synonym Gene Names
BHLHE78; MOP1; PASD8; HIF-1-alpha; HIF1-alpha; bHLHe78
UniProt Entry Name
HIF1A_HUMAN
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Product Notes
The HIF1A hif1a (Catalog #AAA197109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIF1A antibody - N-terminal region reacts with Tested Species Reactivity: Human Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HIF1A hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EGAGGANDKK KISSERRKEK SRDAARSRRS KESEVFYELA HQLPLPHNVS. It is sometimes possible for the material contained within the vial of "HIF1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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