IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embeded human tonsil using at dilution of 1:50.)
Rabbit anti-Human HLA class II histocompatibility antigen, DP beta 1 chain Polyclonal Antibody | anti-HLA-DPB1 antibody
HLA class II histocompatibility antigen, DP beta 1 chain Polyclonal Antibody
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
HLA class II histocompatibility antigen, DP beta 1 chain, Antibody; HLA class II histocompatibility antigen, DP beta 1 chain Polyclonal Antibody; HLA class II histocompatibility antigen; DP(W4) beta chain; MHC class II antigen DPB1; HLA-DPB1; HLA-DP1B; anti-HLA-DPB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total HLA class II histocompatibility antigen, DP beta 1 chain polyclonal antibody.
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
258
Applicable Applications for anti-HLA-DPB1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Human HLA class II histocompatibility antigen, DP beta 1 chain protein
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embeded human tonsil using at dilution of 1:50.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embeded human tonsil using at dilution of 1:50.)
WB (Western Blot)
(All lanes: HLA class II histocompatibility antigen, DP beta 1 chain antibody at 2ug/ml)
WB (Western Blot)
(All lanes: HLA class II histocompatibility antigen, DP beta 1 chain antibody at 2ug/ml)
Related Product Information for anti-HLA-DPB1 antibody
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Product Categories/Family for anti-HLA-DPB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,159 Da
NCBI Official Full Name
HLA class II histocompatibility antigen, DP beta 1 chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DP beta 1
NCBI Official Symbol
HLA-DPB1
NCBI Official Synonym Symbols
DPB1; HLA-DP; HLA-DPB; HLA-DP1B
NCBI Protein Information
HLA class II histocompatibility antigen, DP beta 1 chain
UniProt Protein Name
HLA class II histocompatibility antigen, DP beta 1 chain
UniProt Gene Name
HLA-DPB1
UniProt Synonym Gene Names
HLA-DP1B
UniProt Entry Name
DPB1_HUMAN
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Product Notes
The HLA-DPB1 hla-dpb1 (Catalog #AAA308562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HLA class II histocompatibility antigen, DP beta 1 chain Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA class II histocompatibility antigen, DP beta 1 chain can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HLA-DPB1 hla-dpb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA class II histocompatibility antigen, DP beta 1 chain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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