IHC (Immunohiostchemistry)
(HLA-DPB1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HLA-DPB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human HLA-DPB1 Polyclonal Antibody | anti-HLA-DPB1 antibody
HLA-DPB1 Antibody (Center)
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HLA-DPB1, Antibody; HLA-DPB1 Antibody (Center); HLA class II histocompatibility antigen; DP beta 1 chain; DP(W4) beta chain; MHC class II antigen DPB1; HLA-DPB1; HLA-DP1B; anti-HLA-DPB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HLA-DPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the Central region of human HLA-DPB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
77-105
Sequence Length
258
Applicable Applications for anti-HLA-DPB1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(HLA-DPB1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HLA-DPB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(HLA-DPB1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HLA-DPB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of HLA-DPB1 (arrow) using rabbit polyclonal HLA-DPB1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DPB1 gene.)
WB (Western Blot)
(Western blot analysis of HLA-DPB1 (arrow) using rabbit polyclonal HLA-DPB1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DPB1 gene.)
Related Product Information for anti-HLA-DPB1 antibody
HLA-DPB belongs to the HLA class II beta chain paralogues.
This class II molecule is a heterodimer consisting of an alpha
(DPA) and a beta chain (DPB), both anchored in the membrane. It
plays a central role in the immune system by presenting peptides
derived from extracellular proteins. Class II molecules are
expressed in antigen presenting cells (APC: B lymphocytes,
dendritic cells, macrophages). The beta chain is approximately
26-28 kDa and its gene contains 6 exons. Exon one encodes the
leader peptide, exons 2 and 3 encode the two extracellular domains,
exon 4 encodes the transmembrane domain and exon 5 encodes the
cytoplasmic tail. Within the DP molecule both the alpha chain and
the beta chain contain the polymorphisms specifying the peptide
binding specificities, resulting in up to 4 different molecules.
This class II molecule is a heterodimer consisting of an alpha
(DPA) and a beta chain (DPB), both anchored in the membrane. It
plays a central role in the immune system by presenting peptides
derived from extracellular proteins. Class II molecules are
expressed in antigen presenting cells (APC: B lymphocytes,
dendritic cells, macrophages). The beta chain is approximately
26-28 kDa and its gene contains 6 exons. Exon one encodes the
leader peptide, exons 2 and 3 encode the two extracellular domains,
exon 4 encodes the transmembrane domain and exon 5 encodes the
cytoplasmic tail. Within the DP molecule both the alpha chain and
the beta chain contain the polymorphisms specifying the peptide
binding specificities, resulting in up to 4 different molecules.
Product Categories/Family for anti-HLA-DPB1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Yang, J.H., et al. Tissue Antigens 76(4):282-288(2010)
Wang, P., et al. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24(6):261-263(2010)
Ovsyannikova, I.G., et al. PLoS ONE 5 (7), E11806 (2010) :
Mychaleckyj, J.C., et al. Clin Trials 7 (1 SUPPL), S75-S87 (2010) :
Yang, J.H., et al. Tissue Antigens 76(4):282-288(2010)
Wang, P., et al. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24(6):261-263(2010)
Ovsyannikova, I.G., et al. PLoS ONE 5 (7), E11806 (2010) :
Mychaleckyj, J.C., et al. Clin Trials 7 (1 SUPPL), S75-S87 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29159
NCBI Official Full Name
HLA class II histocompatibility antigen, DP beta 1 chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DP beta 1
NCBI Official Symbol
HLA-DPB1
NCBI Official Synonym Symbols
DPB1; HLA-DP; HLA-DPB; HLA-DP1B
NCBI Protein Information
HLA class II histocompatibility antigen, DP beta 1 chain
UniProt Protein Name
HLA class II histocompatibility antigen, DP beta 1 chain
UniProt Gene Name
HLA-DPB1
UniProt Synonym Gene Names
HLA-DP1B
UniProt Entry Name
DPB1_HUMAN
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Product Notes
The HLA-DPB1 hla-dpb1 (Catalog #AAA288459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 77-105. The HLA-DPB1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA-DPB1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HLA-DPB1 hla-dpb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA-DPB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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