Mouse HLA-DP Antibody | anti-HLA-DPB1 antibody

HLA-DP (MHC II) Mouse Monoclonal Antibody

Cat. Num. AAA13830

• Gene Names: HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• Reactivity: Human. Others not known.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: HLA-DP; N/A; HLA-DP (MHC II) Mouse Monoclonal Antibody; HLA class II histocompatibility antigen DP alpha 1 chain; HLA DP1A; HLA SB alpha chain; HLADP; HLADPA1; HLASB; HLASB histocompatibility type; Human MHC class II HLA SB alpha; Major histocompatibility complex class II DP alpha 1; MHC class II DP3 alpha; MHC class II DPA1; PLT1; Primed lymphocyte test 1; anti-HLA-DPB1 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Isotype: IgG2b, kappa
• Clone Number: SPM421
• Specificity: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-HLA-DPB1 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: ELISA (For coating use Ab at 2-4ug/ml, order antibody without BSA); Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Optimal dilution for a specific application should be determined.
• Human Chromosome Location: 6p21.3
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
• Cellular Localization: Cell surface
• Positive Control: Raji cells. Tonsil or lymph node.
• Preparation and Storage: Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Human Raji cells using HLA-DP (MHC II) Monoclonal Antibody (SPM421) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)

References

Babusikova O; Ujhazy P; Hrivnakova A; Chorvath B; Polakova K. Studies on the distribution of the antigens detected by some newly prepared monoclonal antibodies in normal hemopoietic and leukemic cells. Neoplasma, 1985, 32(6):657-62. Polakova K; Chorvath B; Sedlak J; Duraj J; Matoska J; Karpatova M. Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line. Neoplasma, 1985, 32(6):641-8. Neoplasma 34(4), 417- 425, (1987). Horejsi V, et. al. Tissue Antigens, 1988, 32(1):6-11.

NCBI and Uniprot Product Information

• NCBI GI #: 24797076
• NCBI GeneID: 3115
• NCBI Accession #: NP_002112.3
• NCBI GenBank Nucleotide #: NM_002121.5
• UniProt Accession #: P04440; A0PFJ7; A5I886; A8YPB3; B5U8B4; B7VF80; B7VF87; B8ZX68; B8ZYT0; B9W5S8; B9W6F7; B9W6F9
• Molecular Weight: 33-35 kDa
• NCBI Official Full Name: HLA class II histocompatibility antigen, DP beta 1 chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DP beta 1
• NCBI Official Symbol: HLA-DPB1
• NCBI Official Synonym Symbols: DPB1; HLA-DP; HLA-DPB; HLA-DP1B
• NCBI Protein Information: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DP beta 1 chain
• UniProt Gene Name: HLA-DPB1
• UniProt Synonym Gene Names: HLA-DP1B
• UniProt Entry Name: DPB1_HUMAN