IF (Immunofluorescence)
(Immunofluorescence of HMGB1 in human lung tissue with HMGB1 antibody at 20 μg/mL.)
Rabbit HMGB1 Polyclonal Antibody | anti-HMGB1 antibody
HMGB1 Antibody
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
HMGB1 antibody is affinity chromatography purified via peptide column.
Synonyms
HMGB1, Antibody; HMGB1 Antibody; HMGB1; HMG1; HMG3; SBP-1; High mobility group protein B1; High mobility group protein 1; HMG-1; high mobility group box 1; anti-HMGB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HMGB1 antibody is human, mouse and rat reactive.
Purity/Purification
HMGB1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
215
Applicable Applications for anti-HMGB1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
HMGB1 antibody was raised against a 19 amino acid peptide near the center of human HMGB1.
Buffer
HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of HMGB1 in human lung tissue with HMGB1 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of HMGB1 in human lung tissue with HMGB1 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of HMGB1 in human lung tissue with HMGB1 antibody at 5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of HMGB1 in human lung tissue with HMGB1 antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of HMGB1 in human brain tissue lysate with HMGB1 antibody at (A) 1 and (B) 2 μg/ml)
WB (Western Blot)
(Western blot analysis of HMGB1 in human brain tissue lysate with HMGB1 antibody at (A) 1 and (B) 2 μg/ml)
Related Product Information for anti-HMGB1 antibody
The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2). However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS)-induced endotoxemia and sepsis (3). HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 24 kDa
Observed: 24 kDa
Observed: 24 kDa
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
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Product Notes
The HMGB1 hmgb1 (Catalog #AAA40782) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGB1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HMGB1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the HMGB1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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