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WB (Western Blot)
(Western blot analysis of Phospho-HNF1A(Ser247) in lysates of HepG2?, using Phospho-HNF1A(Ser247) Antibody.)
Rabbit HNF1A Polyclonal Antibody | anti-HNF1A antibody
Phospho-HNF1A (Ser247) Antibody
Gene Names
HNF1A; HNF1; LFB1; TCF1; HNF4A; MODY3; TCF-1; HNF-1A; IDDM20
Reactivity
Human, Mouse, RatPredicted: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
HNF1A, Antibody; Phospho-HNF1A (Ser247) Antibody; Albumin proximal factor; Hepatic nuclear factor 1 alpha; Hepatic nuclear factor 1; Hepatic transcription factor 1 alpha; Hepatic transcription factor 1; Hepatocyte nuclear factor 1-alpha; HNF 1; HNF 1A; HNF-1-alpha; HNF-1A; hnf1a; HNF1A_HUMAN; Interferon production regulator factor; LF B1; LF B1 hepatic nuclear factor; LFB 1; LFB1; LFB1 hepatic nuclear factor; Liver specific transcription factor LF B1; Liver specific transcription factor LFB1; Liver-specific transcription factor LF-B1; Maturity onset diabetes of the young 3; MODY 3; MODY3; TCF 1; TCF-1; TCF1; Transcription factor 1; Transcription factor 1 hepatic; anti-HNF1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Predicted: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-HNF1A(Ser247) Antibody detects endogenous levels of HNF1A only when phosphorylated at Ser247.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
631
Applicable Applications for anti-HNF1A antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human HNF1A around the phosphorylation site of Ser247.
Tissue Specificity
Liver.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-HNF1A(Ser247) in lysates of HepG2?, using Phospho-HNF1A(Ser247) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-HNF1A(Ser247) in lysates of HepG2?, using Phospho-HNF1A(Ser247) Antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemisry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-HNF1A antibody
Description: Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Subunit Structure: Binds DNA as a dimer. Interacts with PCBD1. Heterotetramer with PCBD1; formed by a dimer of dimers.
Similarity: Belongs to the HNF1 homeobox family.
Subunit Structure: Binds DNA as a dimer. Interacts with PCBD1. Heterotetramer with PCBD1; formed by a dimer of dimers.
Similarity: Belongs to the HNF1 homeobox family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 56 kDa
Predicted: 68 kDa
Predicted: 68 kDa
NCBI Official Full Name
hepatocyte nuclear factor 1-alpha isoform 2
NCBI Official Synonym Full Names
HNF1 homeobox A
NCBI Official Symbol
HNF1A
NCBI Official Synonym Symbols
HNF1; LFB1; TCF1; HNF4A; MODY3; TCF-1; HNF-1A; IDDM20
NCBI Protein Information
hepatocyte nuclear factor 1-alpha
UniProt Protein Name
Hepatocyte nuclear factor 1-alpha
UniProt Gene Name
HNF1A
UniProt Synonym Gene Names
TCF1; HNF-1-alpha; HNF-1A; LFB1; TCF-1
UniProt Entry Name
HNF1A_HUMAN
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Product Notes
The HNF1A hnf1a (Catalog #AAA328247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-HNF1A (Ser247) Antibody reacts with Human, Mouse, Rat Predicted: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's HNF1A can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HNF1A hnf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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