Goat HNF4A / HNF4 Polyclonal Antibody | anti-HNF4A antibody

Goat Polyclonal to Human HNF4A / HNF4

Cat. Num. AAA50986

• Gene Names: HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• Reactivity: Human, Monkey, Bat, Dog, Hamster, Horse, Pig, Rabbit.
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified; Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: HNF4A / HNF4, Antibody; Goat Polyclonal to Human HNF4A / HNF4; Anti-HNF4A / HNF4 Antibody (N-Terminus) IHC-plus; HNF4A; Hepatic nuclear factor 4 alpha; HNF4; HNF4a8; NR2A21; TCF; TCF-14; TCF14; MODY1; Transcription factor 14; Transcription factor HNF-4; HNF-4-alpha; HNF4 alpha; HNF4a7; HNF4a9; HNF4alpha; HNF4alpha10/11/12; MODY; NR2A1; Human HNF4A; anti-HNF4A antibody

• Host: Goat
• Reactivity: Human, Monkey, Bat, Dog, Hamster, Horse, Pig, Rabbit.
• Clonality: Polyclonal
• Specificity: Human HNF4A / HNF4. This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1; NP_000448.3; NP_849181.1 resp.).
• Purity/Purification: Immunoaffinity Purified; Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.02% Sodium azide, 0.05% BSA.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 474

• Applicable Applications for anti-HNF4A antibody: IHC (Immunohistochemistry)
• Target: HNF4A/HNF4
• Immunogen Description: Synthetic peptide RLSKTLVDMDMADY-C from the N-terminus of human HNF4A / HNF4 (NP_849180.1; NP_000448.3; NP_849181.1).
• Immunogen Species: HNF4A/HNF4 was raised against Human.
• Immunogen Type: Synthetic peptide
• Immunogen: HNF4A / HNF4 antibody was raised against synthetic peptide RLSKTLVDMDMADY-C from the N-terminus of human HNF4A / HNF4 (NP_849180.1; NP_000448.3; NP_849181.1).
• Specifity: Human Hnf4a/hnf4. This antibody is expected to recognize the reported isoforms a,b, and c, (NP_000448; NP)849181.1 resp).
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and makes no guarantee of the results.; ; ; Tissue Preparation: ; ; Formalin fixation and embedding in paraffin wax.; ; ; >Tissue Sectioning: ; ; Make 4- um section s and place on pre-cleaned and charged microscope slides. Heat in tissue-drying oven for 45 minutes at 60°C . ; ; ; Deparaffinization:; ; Wash slides in 3 changes of xylene.- 5 minutes each @RT.; ; ; Rehydration: ; ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT.; Wash slides in 2 changes of 95% alcohol- minutes each @ RT.; Wash slides in 1 change of 80% alcohol - 3 minutes @RT.; Rinse slides in gentle running distilled water- 5 minutes @RT.; ; Antigen Retrieval; ; Steam slides in 0.01M sodium citrate bufer, pH 6.0 at 99-100°C - 20 minutes.; Remove in 1X TBS with Tween (TBST)- 1 minute @ RT.; Rinse in 1X TBS with Tween (TBST)- 1 minute @ RT.; ; ; Immunostaining:; ; Do not allow tissues to dry at any time during the staining procedure); Apply a universal protein block- 20 minutes @RT; Drain protein block from slides , apply diluted primary antibody- 45 minutes @RT; Rinse slides in 1x TBST - 1 minute @ RT.; Apply a biotinylated secondary antibdoy appropiate for the primary antibody - 30 minutes @ RT; Rinse slides in 1x TBST - 1 minute @ RT.; Apply alkaline phosphatase streptavidin- 30 minutes @ RT.; Rinse slides in 1x TBST - 1 minute @ RT.; Apply alkaline phosphatase chromogen substate - 30 miunutes @RT.; Wash slides in distilled water - 1 minute @ RT.; ; ; Dehytrate; ; (This method should only be used if the chromogen substrate is alcohol insoluble(e.g Vector Red DAB); Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT.; Wash slides in 2 changes of 95% alcohol- 1 minutes each @ RT ; Wash slides in 3 changes of 100% alcohol- 1 minute each @ RT ; Wash slides in 3 changes of xylene- 1 minute each @RT ; Apply coverslip .
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

WB (Western Blot)

(Antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry)

(Anti-HNF4A / HNF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.)

Related Product Information for anti-HNF4A antibody

Hepatocyte nuclear factor 4 alpha (HNF4 alpha), a NR2 Hepatocyte NF4-Like receptor, is a liver-enriched transcription factor that is involved in the expression of liver-specific genes. HNF4 alpha controls the expression of transcription factor HNF1, which is important in the expression of hepatic genes, including those involved in glucose, cholesterol, and fatty acid metabolism.

Product Categories/Family for anti-HNF4A antibody

Family: NHR; Subfamily: NR2 Hepatocyte NF4-like

NCBI and Uniprot Product Information

• NCBI GI #: 31077205
• NCBI GeneID: 3172
• NCBI Accession #: NP_000448.3
• NCBI GenBank Nucleotide #: NM_000457.4
• UniProt Accession #: P41235; O00659; O00723; Q14540; Q5QPB8; Q6B4V5; Q6B4V6; Q6B4V7; Q92653; Q92654; A5JW41; B2RPP8
• Molecular Weight: 43,988 Da
• NCBI Official Full Name: hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
• NCBI Official Synonym Full Names: hepatocyte nuclear factor 4, alpha
• NCBI Official Symbol: HNF4A
• NCBI Official Synonym Symbols: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• NCBI Protein Information: hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1
• UniProt Protein Name: Hepatocyte nuclear factor 4-alpha
• UniProt Gene Name: HNF4A
• UniProt Synonym Gene Names: HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
• UniProt Entry Name: HNF4A_HUMAN